Abstract
Dental caries is a ubiquitous infectious disease with a nearly 100% lifetime prevalence. Rodent caries models are widely used to investigate the etiology, progression and potential prevention or treatment of the disease. To explore the suitability of these models for deeper investigations of intact surface zones during enamel caries, the structures of early-stage carious lesions in rats were characterized and compared with previous reports on white spot enamel lesions in humans. Synchrotron X-ray microcomputed tomography non-destructively mapped demineralization in carious rat molar specimens across a range of caries severity, identifying 52 lesions across the 30 teeth imaged. Of these lesions, 13 were shown to have intact surface zones. Depth profiles of fractional mineral density were qualitatively similar to lesions in human teeth. However, the thickness of the surface zone in the rat model ranges from 10 to 58μm, and is therefore significantly thinner than in human enamel. These results indicate that a fraction of lesions in rat caries possess an intact surface zone and are qualitatively similar to human lesions at the micrometer scale. This suggests that rat caries models may be a suitable analog through which to investigate the structure of surface zone enamel and its role during dental caries.Synchrotron X-ray microtomography non-destructively maps demineralization within carious rat molar enamel. Depth profile analysis and lesion characterization reveals that many rat carious lesions possess intact surface zones that are similar in form but much thinner than those found in human caries.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1056-1064 |
| Number of pages | 9 |
| Journal | Journal of Synchrotron Radiation |
| Volume | 24 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 1 2017 |
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Keywords
- X-ray microtomography
- caries
- surface zones
- white spot enamel lesions
ASJC Scopus subject areas
- Radiation
- Nuclear and High Energy Physics
- Instrumentation
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Characterization of enamel caries lesions in rat molars using synchrotron X-ray microtomography. / Free, R. D.; Derocher, K.; Stock, Stuart R; Keane, Denis T; Scott-Anne, K.; Bowen, W. H.; Joester, Derk.
In: Journal of Synchrotron Radiation, Vol. 24, No. 5, 01.09.2017, p. 1056-1064.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization of enamel caries lesions in rat molars using synchrotron X-ray microtomography
AU - Free, R. D.
AU - Derocher, K.
AU - Stock, Stuart R
AU - Keane, Denis T
AU - Scott-Anne, K.
AU - Bowen, W. H.
AU - Joester, Derk
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Dental caries is a ubiquitous infectious disease with a nearly 100% lifetime prevalence. Rodent caries models are widely used to investigate the etiology, progression and potential prevention or treatment of the disease. To explore the suitability of these models for deeper investigations of intact surface zones during enamel caries, the structures of early-stage carious lesions in rats were characterized and compared with previous reports on white spot enamel lesions in humans. Synchrotron X-ray microcomputed tomography non-destructively mapped demineralization in carious rat molar specimens across a range of caries severity, identifying 52 lesions across the 30 teeth imaged. Of these lesions, 13 were shown to have intact surface zones. Depth profiles of fractional mineral density were qualitatively similar to lesions in human teeth. However, the thickness of the surface zone in the rat model ranges from 10 to 58μm, and is therefore significantly thinner than in human enamel. These results indicate that a fraction of lesions in rat caries possess an intact surface zone and are qualitatively similar to human lesions at the micrometer scale. This suggests that rat caries models may be a suitable analog through which to investigate the structure of surface zone enamel and its role during dental caries.Synchrotron X-ray microtomography non-destructively maps demineralization within carious rat molar enamel. Depth profile analysis and lesion characterization reveals that many rat carious lesions possess intact surface zones that are similar in form but much thinner than those found in human caries.
AB - Dental caries is a ubiquitous infectious disease with a nearly 100% lifetime prevalence. Rodent caries models are widely used to investigate the etiology, progression and potential prevention or treatment of the disease. To explore the suitability of these models for deeper investigations of intact surface zones during enamel caries, the structures of early-stage carious lesions in rats were characterized and compared with previous reports on white spot enamel lesions in humans. Synchrotron X-ray microcomputed tomography non-destructively mapped demineralization in carious rat molar specimens across a range of caries severity, identifying 52 lesions across the 30 teeth imaged. Of these lesions, 13 were shown to have intact surface zones. Depth profiles of fractional mineral density were qualitatively similar to lesions in human teeth. However, the thickness of the surface zone in the rat model ranges from 10 to 58μm, and is therefore significantly thinner than in human enamel. These results indicate that a fraction of lesions in rat caries possess an intact surface zone and are qualitatively similar to human lesions at the micrometer scale. This suggests that rat caries models may be a suitable analog through which to investigate the structure of surface zone enamel and its role during dental caries.Synchrotron X-ray microtomography non-destructively maps demineralization within carious rat molar enamel. Depth profile analysis and lesion characterization reveals that many rat carious lesions possess intact surface zones that are similar in form but much thinner than those found in human caries.
KW - X-ray microtomography
KW - caries
KW - surface zones
KW - white spot enamel lesions
UR - http://www.scopus.com/inward/record.url?scp=85028701659&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85028701659&partnerID=8YFLogxK
U2 - 10.1107/S1600577517008724
DO - 10.1107/S1600577517008724
M3 - Article
VL - 24
SP - 1056
EP - 1064
JO - Journal of Synchrotron Radiation
JF - Journal of Synchrotron Radiation
SN - 0909-0495
IS - 5
ER -