TY - JOUR
T1 - Characterization of heparan sulfate-proteoglycan of glomerular basement membranes
AU - Kanwar, Y. S.
AU - Veis, A.
AU - Kimura, J. H.
AU - Jakubowski, M. L.
PY - 1984
Y1 - 1984
N2 - Native and de novo synthesized heparan sulfate-proteoglycan (HS-PG) of basement membranes from isolated whole glomeruli were characterized. Sepharose CL-6B chromatograms of [35S]sulfate-labeled de novo synthesized HS-PG extracted from whole glomeruli indicated identical molecular weight characteristics to that isolated from purified basement membranes (M(r) of intact HS-PG ≃ 130,000; M(r) of chains ≃ 25,000). Electron microscopic autoradiography showed that almost all radioactive grains were localized to the basement membranes proper. The estimated M(r) of core protein ≃ 18,000. The sedimentation coefficient of native intact HS-PG was 5.56 S, corresponding to a M(r) between 150,000-250,000, a value in accord with gel filtration data on newly synthesized HS-PG. Physicochemical characteristics of HS-PG of native functional basement membranes differed remarkably from that isolated from the basement membrane-producing tumor, Engelbreth-Holm-Swarm sarcoma.
AB - Native and de novo synthesized heparan sulfate-proteoglycan (HS-PG) of basement membranes from isolated whole glomeruli were characterized. Sepharose CL-6B chromatograms of [35S]sulfate-labeled de novo synthesized HS-PG extracted from whole glomeruli indicated identical molecular weight characteristics to that isolated from purified basement membranes (M(r) of intact HS-PG ≃ 130,000; M(r) of chains ≃ 25,000). Electron microscopic autoradiography showed that almost all radioactive grains were localized to the basement membranes proper. The estimated M(r) of core protein ≃ 18,000. The sedimentation coefficient of native intact HS-PG was 5.56 S, corresponding to a M(r) between 150,000-250,000, a value in accord with gel filtration data on newly synthesized HS-PG. Physicochemical characteristics of HS-PG of native functional basement membranes differed remarkably from that isolated from the basement membrane-producing tumor, Engelbreth-Holm-Swarm sarcoma.
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U2 - 10.1073/pnas.81.3.762
DO - 10.1073/pnas.81.3.762
M3 - Article
C2 - 6230667
AN - SCOPUS:0345582659
SN - 0027-8424
VL - 81
SP - 762
EP - 766
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 3 I
ER -
