Abstract
Background: Mechanisms regulating androgen receptor (AR) subcellular localization represent an essential component of AR signaling. Karyopherins are a family of nucleocytoplasmic traficking factors. In this paper, we used the yeast model to study the effects of karyopherins on the subcellular localization of the AR. Methods: Yeast mutants deicient in different nuclear transport factors were transformed with various AR based, GFP tagged constructs and their localization was monitored using microscopy. Results: We showed that yeast can mediate androgen-induced AR nuclear localization and that in addition to the import factor, Importinα/β, this process required the import karyopherin Sxm1. We also showed that a previously identiied nuclear export sequence (NESAR) in the ligand binding domain of AR does not appear to rely on karyopherins for cytoplasmic localization. Conclusions: These results suggest that while AR nuclear import relies on karyopherin activity, AR nuclear export and/or cytoplasmic localization may require other undeined mechanisms.
Original language | English (US) |
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Pages (from-to) | 2768-2779 |
Number of pages | 12 |
Journal | International Journal of Clinical and Experimental Pathology |
Volume | 7 |
Issue number | 6 |
State | Published - 2014 |
Keywords
- Androgen receptor
- Karyopherin
- Nucleocytoplasmic traficking
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology