Characterization of karyopherins in androgen receptor intracellular traficking in the yeast model

Minh M. Nguyen, Robert M. Harmon, Zhou Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: Mechanisms regulating androgen receptor (AR) subcellular localization represent an essential component of AR signaling. Karyopherins are a family of nucleocytoplasmic traficking factors. In this paper, we used the yeast model to study the effects of karyopherins on the subcellular localization of the AR. Methods: Yeast mutants deicient in different nuclear transport factors were transformed with various AR based, GFP tagged constructs and their localization was monitored using microscopy. Results: We showed that yeast can mediate androgen-induced AR nuclear localization and that in addition to the import factor, Importinα/β, this process required the import karyopherin Sxm1. We also showed that a previously identiied nuclear export sequence (NESAR) in the ligand binding domain of AR does not appear to rely on karyopherins for cytoplasmic localization. Conclusions: These results suggest that while AR nuclear import relies on karyopherin activity, AR nuclear export and/or cytoplasmic localization may require other undeined mechanisms.

Original languageEnglish (US)
Pages (from-to)2768-2779
Number of pages12
JournalInternational Journal of Clinical and Experimental Pathology
Volume7
Issue number6
StatePublished - 2014

Keywords

  • Androgen receptor
  • Karyopherin
  • Nucleocytoplasmic traficking

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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