Characterization of Methanobactin from Methylosinus sp. LW4

Grace E. Kenney; Anthony W. Goering; Matthew O. Ross; Caroline J. Dehart; Paul M. Thomas; Brian M. Hoffman; Neil L. Kelleher; Amy C. Rosenzweig

doi:10.1021/jacs.6b06821

Characterization of Methanobactin from Methylosinus sp. LW4

Grace E. Kenney, Anthony W. Goering, Matthew O. Ross, Caroline J. Dehart, Paul M. Thomas, Brian M. Hoffman, Neil L. Kelleher, Amy C. Rosenzweig^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Methanobactins (Mbns) are a growing family of ribosomally produced, post-translationally modified natural products. Characteristic nitrogen-containing heterocycles and neighboring thioamides allow these compounds to bind copper with high affinity. Genome mining has enabled the identification of Mbn operons in bacterial genomes and the prediction of diverse Mbn structures from operon content and precursor peptide sequence. Here we report the characterization of Mbn from Methylosinus (Ms.) species (sp.) LW4. The peptide backbone is distinct from all previously characterized Mbns, and the post-translational modifications correspond precisely to those predicted on the basis of the Ms. sp. LW4 Mbn operon. Thus, prediction based on genome analysis combined with isolation and structural characterization represents a phylogenetic approach to finding diverse Mbns and elucidating their biosynthetic pathways.

Original language	English (US)
Pages (from-to)	11124-11127
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	138
Issue number	35
DOIs	https://doi.org/10.1021/jacs.6b06821
State	Published - Sep 7 2016

Funding

IMSERC is supported by NIH grants 1S10OD012016-01/1S10RR019071-01A1, Northwestern University/the state of Illinois, the IIN and the NSF under NNCI-1542205. High-resolution mass spectral analyses were partially supported by the NRTDP (GM108569). Funding for this work was provided by the NSF (MCB0842366 to A.C.R.), the NIH (GM070473 and GM118035 to A.C.R., GM111097 to B.M.H., AT009143 and GM108569 to N.L.K., 5T32GM008382 to M.O.R.), and the AHA (14PRE20460104 to G.E.K.).

ASJC Scopus subject areas

General Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry

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10.1021/jacs.6b06821

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title = "Characterization of Methanobactin from Methylosinus sp. LW4",

abstract = "Methanobactins (Mbns) are a growing family of ribosomally produced, post-translationally modified natural products. Characteristic nitrogen-containing heterocycles and neighboring thioamides allow these compounds to bind copper with high affinity. Genome mining has enabled the identification of Mbn operons in bacterial genomes and the prediction of diverse Mbn structures from operon content and precursor peptide sequence. Here we report the characterization of Mbn from Methylosinus (Ms.) species (sp.) LW4. The peptide backbone is distinct from all previously characterized Mbns, and the post-translational modifications correspond precisely to those predicted on the basis of the Ms. sp. LW4 Mbn operon. Thus, prediction based on genome analysis combined with isolation and structural characterization represents a phylogenetic approach to finding diverse Mbns and elucidating their biosynthetic pathways.",

author = "Kenney, {Grace E.} and Goering, {Anthony W.} and Ross, {Matthew O.} and Dehart, {Caroline J.} and Thomas, {Paul M.} and Hoffman, {Brian M.} and Kelleher, {Neil L.} and Rosenzweig, {Amy C.}",

note = "Funding Information: IMSERC is supported by NIH grants 1S10OD012016-01/1S10RR019071-01A1, Northwestern University/the state of Illinois, the IIN and the NSF under NNCI-1542205. High-resolution mass spectral analyses were partially supported by the NRTDP (GM108569). Funding for this work was provided by the NSF (MCB0842366 to A.C.R.), the NIH (GM070473 and GM118035 to A.C.R., GM111097 to B.M.H., AT009143 and GM108569 to N.L.K., 5T32GM008382 to M.O.R.), and the AHA (14PRE20460104 to G.E.K.). Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

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AU - Thomas, Paul M.

AU - Hoffman, Brian M.

AU - Kelleher, Neil L.

AU - Rosenzweig, Amy C.

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N2 - Methanobactins (Mbns) are a growing family of ribosomally produced, post-translationally modified natural products. Characteristic nitrogen-containing heterocycles and neighboring thioamides allow these compounds to bind copper with high affinity. Genome mining has enabled the identification of Mbn operons in bacterial genomes and the prediction of diverse Mbn structures from operon content and precursor peptide sequence. Here we report the characterization of Mbn from Methylosinus (Ms.) species (sp.) LW4. The peptide backbone is distinct from all previously characterized Mbns, and the post-translational modifications correspond precisely to those predicted on the basis of the Ms. sp. LW4 Mbn operon. Thus, prediction based on genome analysis combined with isolation and structural characterization represents a phylogenetic approach to finding diverse Mbns and elucidating their biosynthetic pathways.

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Characterization of Methanobactin from Methylosinus sp. LW4

Abstract

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