Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group

Jeremie B. Fant; Mereida Fluckes; Evana James; Hilary Noble; Jordan Wood

doi:10.1002/aps3.11303

Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group

Jeremie B. Fant^*, Mereida Fluckes, Evana James, Hilary Noble, Jordan Wood

^*Corresponding author for this work

Program in Biological Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Premise: Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae). Methods and Results: Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross-species amplification in an additional 13 taxa that represented all six genera within the Hawai‘ian lobelioid group to determine the broader applicability of the markers. Conclusions: Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both Brighamia species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai‘ian lobelioid group.

Original language	English (US)
Article number	e11303
Journal	Applications in Plant Sciences
Volume	7
Issue number	11
DOIs	https://doi.org/10.1002/aps3.11303
State	Published - Nov 1 2019

Funding

The authors thank the Eppley Foundation, the Institute of Museum and Library Services (IMLS; MG-30-16-0085-16), The Chicago Botanic Garden College First Program, the\u00A0Charles and Margery Barancik Foundation, and the U.S. National Science Foundation Research Experiences for Undergraduates (NSF-REU) program (DEB-1757800) for funding. We are grateful to Nick Harvey of Genetic Services UK for developing the markers. We also thank Seana Walsh and the National Tropical Botanic Garden for providing samples, Laura Steger for help in the lab, and Kay Havens and Andrea Kramer for reviewing the manuscript.

Keywords

Brighamia
Campanulaceae
Hawai‘ian lobelioids
Lobelia
cross-amplification
microsatellites

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Plant Science

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10.1002/aps3.11303

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title = "Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai{\textquoteleft}ian lobelioid group",

abstract = "Premise: Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae). Methods and Results: Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross-species amplification in an additional 13 taxa that represented all six genera within the Hawai{\textquoteleft}ian lobelioid group to determine the broader applicability of the markers. Conclusions: Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both Brighamia species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai{\textquoteleft}ian lobelioid group.",

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author = "Fant, {Jeremie B.} and Mereida Fluckes and Evana James and Hilary Noble and Jordan Wood",

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AU - Fant, Jeremie B.

AU - Fluckes, Mereida

AU - James, Evana

AU - Noble, Hilary

AU - Wood, Jordan

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Premise: Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae). Methods and Results: Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross-species amplification in an additional 13 taxa that represented all six genera within the Hawai‘ian lobelioid group to determine the broader applicability of the markers. Conclusions: Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both Brighamia species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai‘ian lobelioid group.

AB - Premise: Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae). Methods and Results: Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross-species amplification in an additional 13 taxa that represented all six genera within the Hawai‘ian lobelioid group to determine the broader applicability of the markers. Conclusions: Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both Brighamia species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai‘ian lobelioid group.

KW - Brighamia

KW - Campanulaceae

KW - Hawai‘ian lobelioids

KW - Lobelia

KW - cross-amplification

KW - microsatellites

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Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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