Characterization of murine CD3+ TCRαβ+ CD4- CD8- thymocytes

Tian Tian, Ling Gao, Xiaoping Qian, Shunyou Gong, Weifeng Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective To analyze the characteristics of murine CD3+ TCRαβ+ DN thymocytes and deduce their phenotypic and functional process in the thymus. Methods Murine DN thymocytes were purified and multi-color staining was used to analyze the phenotype and TCR repertoire of CD3+ TCRαβ+ DN cells. Results DN thymocytes are heterogeneous containing both CD3- DN and CD3+ DN cells. CD3+ DN thymocytes can also be classified into two subsets as CD3+ TCRαβ+ and CD3+ TCRγδ+ . CD3+ TCRαβ+ DN thymocytes are small and most of them are cortisone resistant. The percentage of self-reactive Vβ3+ and Vβ11+ among these cells is low. The phenotype of CD3+ TCRαβ+ DN thymocytes is quite mature, correspondence to that of medullary type SP thymocytes. Conclusions Unlike CD3- TCRαβ- DN thymocytes, CD3+ TCRαβ+ DN thymocyte population is a unique subset. They will undergo a phenotypic and functional maturation process before leaving thymus and entering into periphery zone.

Original languageEnglish (US)
Pages (from-to)316-320
Number of pages5
JournalChinese Journal of Microbiology and Immunology
Issue number3
StatePublished - May 2002


  • CD4 CD8
  • Phenotype
  • TCR repertoire
  • Thymocytes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology


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