Characterization of murine CD3 + TCRαβ + CD4 - CD8 - thymocytes

Tian Tian, Ling Gao, Xiaoping Qian, Shunyou Gong, Weifeng Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objective To analyze the characteristics of murine CD3 + TCRαβ + DN thymocytes and deduce their phenotypic and functional process in the thymus. Methods Murine DN thymocytes were purified and multi-color staining was used to analyze the phenotype and TCR repertoire of CD3 + TCRαβ + DN cells. Results DN thymocytes are heterogeneous containing both CD3 - DN and CD3 + DN cells. CD3 + DN thymocytes can also be classified into two subsets as CD3 + TCRαβ + and CD3 + TCRγδ + . CD3 + TCRαβ + DN thymocytes are small and most of them are cortisone resistant. The percentage of self-reactive Vβ3 + and Vβ11 + among these cells is low. The phenotype of CD3 + TCRαβ + DN thymocytes is quite mature, correspondence to that of medullary type SP thymocytes. Conclusions Unlike CD3 - TCRαβ - DN thymocytes, CD3 + TCRαβ + DN thymocyte population is a unique subset. They will undergo a phenotypic and functional maturation process before leaving thymus and entering into periphery zone.

Original languageEnglish (US)
Pages (from-to)316-320
Number of pages5
JournalChinese Journal of Microbiology and Immunology
Volume22
Issue number3
StatePublished - May 1 2002

Keywords

  • CD4 CD8
  • Phenotype
  • TCR repertoire
  • Thymocytes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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