Characterization of nuclear and chromatin poly A-containing RNA.

S. Sakamoto*, M. S. Rao, B. Wu, W. H. Spohn, H. Busch

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Poly A-containing RNA was isolated from Novikoff hepatoma cell chromatin on nitrocellulose membrane filters after the cells were labeled with 32P-orthophosphate for 6 hours. Under denaturing conditions, the poly A-containing chromatin RNA had a sedimentation coefficient of approximately 8-18S. The presence of poly A tracts in the chromatin RNA fractions indicate that poly A addition occurs almost immediately after transcription. Poly A-containing chromatin RNA and nuclear RNA were approximately 1/5 as active as cytoplasmic poly A-containing RNA in the wheat germ translational system. Since chromatin poly A-containing RNA is the precursor of cytoplasmic poly A-containing RNA, further processing of this RNA prior to its attachment to polysomes may account for its increase in translational capacity.

Original languageEnglish (US)
Pages (from-to)309-324
Number of pages16
JournalPhysiological chemistry and physics
Volume7
Issue number4
StatePublished - Dec 1 1975

ASJC Scopus subject areas

  • Medicine(all)

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