TY - JOUR
T1 - Characterization of the bout durations of sleep and wakefulness
AU - McShane, Blakeley B.
AU - Galante, Raymond J.
AU - Jensen, Shane T.
AU - Naidoo, Nirinjini
AU - Pack, Allan I.
AU - Wyner, Abraham
N1 - Funding Information:
We are grateful to Mr. Daniel Barrett and Ms. Jennifer Montoya for help in the preparation of this manuscript. Original research was supported by NIH Program Project Grant AG-17628 and HL07953 .
PY - 2010/11/30
Y1 - 2010/11/30
N2 - Study objectives: (a) Develop a new statistical approach to describe the microarchitecture of wakefulness and sleep in mice; (b) evaluate differences among inbred strains in this microarchitecture; (c) compare results when data are scored in 4-s versus 10-s epochs. Design: Studies in male mice of four inbred strains: AJ, C57BL/6, DBA and PWD. EEG/EMG were recorded for 24. h and scored independently in 4-s and 10-s epochs. Measurements and results: Distribution of bout durations of wakefulness, NREM and REM sleep in mice has two distinct components, i.e., short and longer bouts. This is described as a spike (short bouts) and slab (longer bouts) distribution, a particular type of mixture model. The distribution in any state depends on the state the mouse is transitioning from and can be characterized by three parameters: the number of such bouts conditional on the previous state, the size of the spike, and the average length of the slab. While conventional statistics such as time spent in state, average bout duration, and number of bouts show some differences between inbred strains, this new statistical approach reveals more major differences. The major difference between strains is their ability to sustain long bouts of NREM sleep or wakefulness. Scoring mouse sleep/wake in 4-s epochs offered little new information when using conventional metrics but did when evaluating the microarchitecture based on this new approach. Conclusions: Standard statistical approaches do not adequately characterize the microarchitecture of mouse behavioral state. Approaches based on a spike-and-slab provide a quantitative description.
AB - Study objectives: (a) Develop a new statistical approach to describe the microarchitecture of wakefulness and sleep in mice; (b) evaluate differences among inbred strains in this microarchitecture; (c) compare results when data are scored in 4-s versus 10-s epochs. Design: Studies in male mice of four inbred strains: AJ, C57BL/6, DBA and PWD. EEG/EMG were recorded for 24. h and scored independently in 4-s and 10-s epochs. Measurements and results: Distribution of bout durations of wakefulness, NREM and REM sleep in mice has two distinct components, i.e., short and longer bouts. This is described as a spike (short bouts) and slab (longer bouts) distribution, a particular type of mixture model. The distribution in any state depends on the state the mouse is transitioning from and can be characterized by three parameters: the number of such bouts conditional on the previous state, the size of the spike, and the average length of the slab. While conventional statistics such as time spent in state, average bout duration, and number of bouts show some differences between inbred strains, this new statistical approach reveals more major differences. The major difference between strains is their ability to sustain long bouts of NREM sleep or wakefulness. Scoring mouse sleep/wake in 4-s epochs offered little new information when using conventional metrics but did when evaluating the microarchitecture based on this new approach. Conclusions: Standard statistical approaches do not adequately characterize the microarchitecture of mouse behavioral state. Approaches based on a spike-and-slab provide a quantitative description.
KW - Genetics of behavior
KW - Inbred mouse strains
KW - Mouse sleep
KW - REM sleep
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U2 - 10.1016/j.jneumeth.2010.08.024
DO - 10.1016/j.jneumeth.2010.08.024
M3 - Article
C2 - 20817037
AN - SCOPUS:78049358296
SN - 0165-0270
VL - 193
SP - 321
EP - 333
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 2
ER -