Characterization of the cDNA corresponding to a phosphoprotein (p43) intermediary in the action of ACTH

Carla Finkielstein*, Cora Cymeryng, Cristina Paz, Isabel Neuman, Laura Dada, Fabiana Cornejo Maciel, Pablo G. Mele, Carlos F. Mendez, Paula Maloberti, Angela R. Solano, Bernard P. Schimmer, Ernesto J. Podestá

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We have previously isolated and partially-sequenced a soluble phosphoprotein (p43) that acts as intermediary in the stimulation of steroid synthesis. In this report we have used synthetic peptides whose sequences match those obtained from p43 to generate antipeptide antibodies and show that these antibodies bind to purified p43 protein as determined by immunoblot analysis. The presence of p43 was detected by Western blot in both steroidogenic and non-steroidogenic tissues. One of the antibodies was also used to purify p43 on immunoaffinity chromatography columns. Proteins eluting from affinity columns produce a twelve-fold stimulation of progesterone synthesis. This effect was blocked by the use of an inhibitor of phospholipase A2. These results suggest the involvement of p43 in transducing the adrenocorticotropin signal to mitochondria in zona fasciculata cells. We also describe a partial cDNA clone with a predicted amino acid sequence that matches the sequences of the internal peptides of p43.

Original languageEnglish (US)
Pages (from-to)521-532
Number of pages12
JournalEndocrine Research
Volume22
Issue number4
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Endocrinology

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