Characterization of the menaquinone-dependent disulfide bond formation pathway of Escherichia coli

Yohhei Takahashi, Kenji Inaba, Koreaki Ito*

*Corresponding author for this work

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

In the protein disulfide-introducing system of Escherichia coli, plasma membrane-integrated DsbB oxidizes periplasmic BsbA, the primary disulfide donor. Whereas the DsbA-BsbB system utilizes the oxidizing power of ubiquinone (UQ) under aerobic conditions, menaquinone (MK) is believed to function as an immediate electron acceptor under anaerobic conditions. Here, we characterized MK reactivities with DsbB. In the absence of UQ, DsbB was complexed with MK8 in the cell. In vitro studies showed that, by binding to DsbB in a manner competitive with UQ, MK specifically oxidized Cys41 and Cys 44 of DsbB and activated its catalytic function to oxidize reduced DsbA. In contrast, menadione used in earlier studies proved to be a more nonspecific oxidant of DsbB. During catalysis, MK8 underwent a spectroscopic transition to develop a visible violet color (λmax = 550 nm), which required a reduced state of Cys44 as shown previously for UQ color development (λmax = 500 nm) on DsbB. In an in vitro reaction system of MK8-dependent oxidation of DsbA at 30°C, two reaction components were observed, one completing within minutes and the other taking > 1 h. Both of these reaction modes were accompanied by the transition state of MK, for which the slower reaction proceeded through the disulfide-linked DsbA-DsbB(MK) intermediate. The MK-dependent pathway provides opportunities to further dissect the quinone-dependent DsbA-DsbB redox reactions.

Original languageEnglish (US)
Pages (from-to)47057-47065
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number45
DOIs
StatePublished - Nov 5 2004

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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