Characterization of the mycoplasma pneumoniae proteome with 100% sequence coverage by high resolution MS/MS

Leah M. Miller*, Yi Du, Jeffrey R. Johnson, Fanyu Meng, Gregory K. Taylor, Neu L. Kelleher

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Proteome with 100% sequence coverage was characterized from Mycoplasma Pneumoniae by using high resolution Mass spectroscopy (MS/MS). It is observed that there were about 500 predicted proteins in M. Pneumoniae, below a mass of 50 kDa. It was found that about 70 fractions was yielded, each containing ∼15-30 proteins with 10 kDa of one another, by the fractionation of M.Pneumoniae lystae. The results show that fractions containing ∼2-10 proteins are obtained from the further fractionation and subsequent acidification of the samples on NPS column.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages167-168
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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