Characterization of the mycoplasma pneumoniae proteome with 100% sequence coverage by high resolution MS/MS

Leah M. Miller; Yi Du; Jeffrey R. Johnson; Fanyu Meng; Gregory K. Taylor; Neu L. Kelleher

Characterization of the mycoplasma pneumoniae proteome with 100% sequence coverage by high resolution MS/MS

Leah M. Miller^*, Yi Du, Jeffrey R. Johnson, Fanyu Meng, Gregory K. Taylor, Neu L. Kelleher

^*Corresponding author for this work

Molecular Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Proteome with 100% sequence coverage was characterized from Mycoplasma Pneumoniae by using high resolution Mass spectroscopy (MS/MS). It is observed that there were about 500 predicted proteins in M. Pneumoniae, below a mass of 50 kDa. It was found that about 70 fractions was yielded, each containing ∼15-30 proteins with 10 kDa of one another, by the fractionation of M.Pneumoniae lystae. The results show that fractions containing ∼2-10 proteins are obtained from the further fractionation and subsequent acidification of the samples on NPS column.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	167-168
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

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@inproceedings{41f9d581d6c743adb89426c29e2adffb,

title = "Characterization of the mycoplasma pneumoniae proteome with 100% sequence coverage by high resolution MS/MS",

abstract = "Proteome with 100% sequence coverage was characterized from Mycoplasma Pneumoniae by using high resolution Mass spectroscopy (MS/MS). It is observed that there were about 500 predicted proteins in M. Pneumoniae, below a mass of 50 kDa. It was found that about 70 fractions was yielded, each containing ∼15-30 proteins with 10 kDa of one another, by the fractionation of M.Pneumoniae lystae. The results show that fractions containing ∼2-10 proteins are obtained from the further fractionation and subsequent acidification of the samples on NPS column.",

author = "Miller, {Leah M.} and Yi Du and Johnson, {Jeffrey R.} and Fanyu Meng and Taylor, {Gregory K.} and Kelleher, {Neu L.}",

year = "2002",

month = dec,

day = "1",

language = "English (US)",

pages = "167--168",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Miller, LM, Du, Y, Johnson, JR, Meng, F, Taylor, GK & Kelleher, NL 2002, Characterization of the mycoplasma pneumoniae proteome with 100% sequence coverage by high resolution MS/MS. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 167-168, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

Characterization of the mycoplasma pneumoniae proteome with 100% sequence coverage by high resolution MS/MS. / Miller, Leah M.; Du, Yi; Johnson, Jeffrey R.; Meng, Fanyu; Taylor, Gregory K.; Kelleher, Neu L.

Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 167-168.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Characterization of the mycoplasma pneumoniae proteome with 100% sequence coverage by high resolution MS/MS

AU - Miller, Leah M.

AU - Du, Yi

AU - Johnson, Jeffrey R.

AU - Meng, Fanyu

AU - Taylor, Gregory K.

AU - Kelleher, Neu L.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Proteome with 100% sequence coverage was characterized from Mycoplasma Pneumoniae by using high resolution Mass spectroscopy (MS/MS). It is observed that there were about 500 predicted proteins in M. Pneumoniae, below a mass of 50 kDa. It was found that about 70 fractions was yielded, each containing ∼15-30 proteins with 10 kDa of one another, by the fractionation of M.Pneumoniae lystae. The results show that fractions containing ∼2-10 proteins are obtained from the further fractionation and subsequent acidification of the samples on NPS column.

AB - Proteome with 100% sequence coverage was characterized from Mycoplasma Pneumoniae by using high resolution Mass spectroscopy (MS/MS). It is observed that there were about 500 predicted proteins in M. Pneumoniae, below a mass of 50 kDa. It was found that about 70 fractions was yielded, each containing ∼15-30 proteins with 10 kDa of one another, by the fractionation of M.Pneumoniae lystae. The results show that fractions containing ∼2-10 proteins are obtained from the further fractionation and subsequent acidification of the samples on NPS column.

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EP - 168

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

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Y2 - 2 June 2002 through 6 June 2002

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