Abstract
Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property of a Cy s2His2-type transcription factor, zinc finger protein 191 (Zfpl91), which has been newly found to play a significant role in mice. By constructing a fusion protein containingthe DNA-binding domain of Zfpl91, we characterized target DNA by determining the protein's binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfpl91 can specifically bind to the TCAT repeat motif and that there is a cooperative effect amongthe target DNA's multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfpl91 in gene regulation and development.
Original language | English (US) |
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Pages (from-to) | 704-710 |
Number of pages | 7 |
Journal | Acta biochimica et biophysica Sinica |
Volume | 40 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2008 |
Keywords
- DNA-binding protein
- Microsatellite
- Zinc finger protein 191
ASJC Scopus subject areas
- General Medicine