Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191

Haoyue Wang, Ruilin Sun, Guoxiang Liu, Minghui Yao, Jian Fei*, Hebai Shen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property of a Cy s2His2-type transcription factor, zinc finger protein 191 (Zfpl91), which has been newly found to play a significant role in mice. By constructing a fusion protein containingthe DNA-binding domain of Zfpl91, we characterized target DNA by determining the protein's binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfpl91 can specifically bind to the TCAT repeat motif and that there is a cooperative effect amongthe target DNA's multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfpl91 in gene regulation and development.

Original languageEnglish (US)
Pages (from-to)704-710
Number of pages7
JournalActa biochimica et biophysica Sinica
Volume40
Issue number8
DOIs
StatePublished - Aug 2008

Keywords

  • DNA-binding protein
  • Microsatellite
  • Zinc finger protein 191

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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