Characterization of the Yersinia enterocolitica type III secretion ATPase YscN and its regulator, YscL

Bill Blaylock, Kelly E. Riordan, Dominique M. Missiakas, Olaf Schneewind*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Type III secretion is a mechanism used by a broad range of gram-negative bacteria to neutralize eukaryotic defenses by enabling translocation of bacterial proteins directly into the cytoplasm of host cells. The bacterial energy source for secretion is ATP, which is consumed by an ATPase that couples ATP hydrolysis to the unfolding of secreted proteins and the dissociation of their chaperones just prior to secretion. By studying the biochemical properties of YscN and YscL of Yersinia enterocolitica, we have characterized them as the ATPase and ATPase regulator, respectively, of the type III secretion system of this organism. In vivo, YscL and YscN interact with each other, and the overexpression of glutathione S-transferase-YscL abolishes secretion and down-regulates the expression of secretion apparatus components.

Original languageEnglish (US)
Pages (from-to)3525-3534
Number of pages10
JournalJournal of bacteriology
Volume188
Issue number10
DOIs
StatePublished - May 2006

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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