Characterization of virus infectivity and cell-free capsid assembly of SIVMneCL8

Julia E. Dooher, Mario Javier Pineda, Julie Overbaugh, Jaisri R. Lingappa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We have previously described a cell-free system that reconstitutes immature capsid assembly of Gag polypeptides from viruses belonging to three major primate lentiviral lineages, including HIV-1, HIV-2 and SIVagm. Studies described here examine a member of the SIVmac/Mne lineage, SIVMneCL8, using assays for virus production and infectivity as well as cellular events in capsid formation. We report that SIVMneCL8, a molecular clone with properties typical of transmitted viral variants, is less infectious per unit p27 Gag than another member of the SIVmac/Mne lineage, SIVmac239. SIVMneCL8 Gag polypeptides are arrested at an early stage of capsid assembly in the cell-free system. Additionally, SIVMneCL8 Gag polypeptides associate minimally with the host factor human HP68. This is the first report of a primate lentivirus that does not complete capsid assembly in the cell-free system.

Original languageEnglish (US)
Pages (from-to)262-271
Number of pages10
JournalJournal of Medical Primatology
Volume33
Issue number5-6
DOIs
StatePublished - Oct 2004

Keywords

  • Gag
  • Macaca nemestrina
  • SIVMne
  • Simian immunodeficiency virus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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