TY - JOUR
T1 - Characterization of virus infectivity and cell-free capsid assembly of SIVMneCL8
AU - Dooher, Julia E.
AU - Pineda, Mario Javier
AU - Overbaugh, Julie
AU - Lingappa, Jaisri R.
PY - 2004/10
Y1 - 2004/10
N2 - We have previously described a cell-free system that reconstitutes immature capsid assembly of Gag polypeptides from viruses belonging to three major primate lentiviral lineages, including HIV-1, HIV-2 and SIVagm. Studies described here examine a member of the SIVmac/Mne lineage, SIVMneCL8, using assays for virus production and infectivity as well as cellular events in capsid formation. We report that SIVMneCL8, a molecular clone with properties typical of transmitted viral variants, is less infectious per unit p27 Gag than another member of the SIVmac/Mne lineage, SIVmac239. SIVMneCL8 Gag polypeptides are arrested at an early stage of capsid assembly in the cell-free system. Additionally, SIVMneCL8 Gag polypeptides associate minimally with the host factor human HP68. This is the first report of a primate lentivirus that does not complete capsid assembly in the cell-free system.
AB - We have previously described a cell-free system that reconstitutes immature capsid assembly of Gag polypeptides from viruses belonging to three major primate lentiviral lineages, including HIV-1, HIV-2 and SIVagm. Studies described here examine a member of the SIVmac/Mne lineage, SIVMneCL8, using assays for virus production and infectivity as well as cellular events in capsid formation. We report that SIVMneCL8, a molecular clone with properties typical of transmitted viral variants, is less infectious per unit p27 Gag than another member of the SIVmac/Mne lineage, SIVmac239. SIVMneCL8 Gag polypeptides are arrested at an early stage of capsid assembly in the cell-free system. Additionally, SIVMneCL8 Gag polypeptides associate minimally with the host factor human HP68. This is the first report of a primate lentivirus that does not complete capsid assembly in the cell-free system.
KW - Gag
KW - Macaca nemestrina
KW - SIVMne
KW - Simian immunodeficiency virus
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U2 - 10.1111/j.1600-0684.2004.00074.x
DO - 10.1111/j.1600-0684.2004.00074.x
M3 - Article
C2 - 15525327
AN - SCOPUS:5744241665
SN - 0047-2565
VL - 33
SP - 262
EP - 271
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
IS - 5-6
ER -
