Characterization of virus-specific T-cell immunity in liver allograft recipients with HCV-induced cirrhosis

A. Bharat, F. Barros, K. Narayanan, B. Borg, M. Lisker-Melman, S. Shenoy, J. Lowell, J. Crippin, W. Chapman, T. Mohanakumar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Recurrent hepatitis C infection (HCV) following liver transplantation causes accelerated allograft cirrhosis. Here we characterized HCV-specific immunity in adult liver transplant recipients (n = 74) with and without allograft cirrhosis. Patients were divided into hepatic inflammation/no cirrhosis (METAVIR scores 0-2, HIN) and hepatic cirrhosis (score 3-4, HFC). As control, 20 normal subjects and 10 non-HCV liver transplant patients were included. Twenty-five different serum cytokines were analyzed using LUMINEX. Frequency of T-cells specific to HCV-derived proteins (NS3, NS4, NS5, Core) was characterized using ELISPOT immunoassays. There was no difference in clinical characteristics between HIN (n = 49) and HFC (n = 25) groups. HIN group had high serum IFN-γ and IL-12 while HFC demonstrated elevated IL-4, IL-5 and IL-10 (p < 0.01). HCV (NS3, NS4, NS5, Core)-specific IFN-γ-producing CD4+ T-cells were elevated in the HIN group whereas the HFC patients showed predominance of HCV-specific IL-5 and IL-10-producing CD4+ T-cells. Conclusions: Lack of HCV-specific Th1-type T-cell immunity is observed in liver transplant recipients with advanced allograft cirrhosis.

Original languageEnglish (US)
Pages (from-to)1214-1220
Number of pages7
JournalAmerican Journal of Transplantation
Volume8
Issue number6
DOIs
StatePublished - Jun 2008

Keywords

  • HCV
  • Immunity
  • Liver allograft cirrhosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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