Characterization of voltage-sensitive calcium channels in a clonal pituitary cell line

Gary A. Shangold*, Sathapana Kongsamut, Richard J. Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We have pharmacologically characterized voltage sensitive calcium channels (VSCCs) in GH3 cells, an anterior pituitary clonal cell line known to secrete prolactin and growth hormone. Raising the medium K+ concentration from 5 to 50 mM caused an immediate increase in net 45Ca2+ uptake which remained apparent over a 15 minute time course. 45Ca2+ uptake was maximally stimulated nearly 10-fold over basal levels. This K+-induced stimulation of Ca2+ uptake was not prevented by 10-5M tetrodotoxin or by replacing sodium with choline in the assay medium. Ca2+ uptake was, however, inhibited by several VSCC antagonists: nitrendipine, D-600, diltiazem and Cd2+. Further, the novel dihydropyridine VSCC agonists, BAY K8644 and CGP 28392, enhanced 50 mM K+-stimulated 45Ca2+ uptake and these effects were blocked by nitrendipine.

Original languageEnglish (US)
Pages (from-to)2209-2215
Number of pages7
JournalLife Sciences
Issue number23
StatePublished - Jun 10 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Characterization of voltage-sensitive calcium channels in a clonal pituitary cell line'. Together they form a unique fingerprint.

Cite this