Characterization of voltage-sensitive calcium channels in a clonal pituitary cell line

Gary A. Shangold; Sathapana Kongsamut; Richard J. Miller

doi:10.1016/0024-3205(85)90331-5

Characterization of voltage-sensitive calcium channels in a clonal pituitary cell line

Gary A. Shangold^*, Sathapana Kongsamut, Richard J. Miller

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We have pharmacologically characterized voltage sensitive calcium channels (VSCCs) in GH₃ cells, an anterior pituitary clonal cell line known to secrete prolactin and growth hormone. Raising the medium K⁺ concentration from 5 to 50 mM caused an immediate increase in net ⁴⁵Ca²⁺ uptake which remained apparent over a 15 minute time course. ⁴⁵Ca²⁺ uptake was maximally stimulated nearly 10-fold over basal levels. This K⁺-induced stimulation of Ca²⁺ uptake was not prevented by 10^-5M tetrodotoxin or by replacing sodium with choline in the assay medium. Ca²⁺ uptake was, however, inhibited by several VSCC antagonists: nitrendipine, D-600, diltiazem and Cd²⁺. Further, the novel dihydropyridine VSCC agonists, BAY K8644 and CGP 28392, enhanced 50 mM K⁺-stimulated ⁴⁵Ca²⁺ uptake and these effects were blocked by nitrendipine.

Original language	English (US)
Pages (from-to)	2209-2215
Number of pages	7
Journal	Life Sciences
Volume	36
Issue number	23
DOIs	https://doi.org/10.1016/0024-3205(85)90331-5
State	Published - Jun 10 1985

Funding

The authors would like to thank Ellsabeth Cornelison for excellent technical asslstance and Della Akres and Joyce Bonner for preparation of the manuscrlpt. This study was supported by NIH grants DA-02121 and DA-02575 to R.J.M. and a grant from The Mothers' Aid Research Fund of Chlcago Lylng-ln Hospital to G.A.S.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1016/0024-3205(85)90331-5

Cite this

@article{67e71b9c75e1447dbe41e574088b344a,

title = "Characterization of voltage-sensitive calcium channels in a clonal pituitary cell line",

abstract = "We have pharmacologically characterized voltage sensitive calcium channels (VSCCs) in GH3 cells, an anterior pituitary clonal cell line known to secrete prolactin and growth hormone. Raising the medium K+ concentration from 5 to 50 mM caused an immediate increase in net 45Ca2+ uptake which remained apparent over a 15 minute time course. 45Ca2+ uptake was maximally stimulated nearly 10-fold over basal levels. This K+-induced stimulation of Ca2+ uptake was not prevented by 10-5M tetrodotoxin or by replacing sodium with choline in the assay medium. Ca2+ uptake was, however, inhibited by several VSCC antagonists: nitrendipine, D-600, diltiazem and Cd2+. Further, the novel dihydropyridine VSCC agonists, BAY K8644 and CGP 28392, enhanced 50 mM K+-stimulated 45Ca2+ uptake and these effects were blocked by nitrendipine.",

author = "Shangold, {Gary A.} and Sathapana Kongsamut and Miller, {Richard J.}",

note = "Funding Information: The authors would like to thank Ellsabeth Cornelison for excellent technical asslstance and Della Akres and Joyce Bonner for preparation of the manuscrlpt. This study was supported by NIH grants DA-02121 and DA-02575 to R.J.M. and a grant from The Mothers' Aid Research Fund of Chlcago Lylng-ln Hospital to G.A.S.",

year = "1985",

month = jun,

day = "10",

doi = "10.1016/0024-3205(85)90331-5",

language = "English (US)",

volume = "36",

pages = "2209--2215",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "23",

}

TY - JOUR

T1 - Characterization of voltage-sensitive calcium channels in a clonal pituitary cell line

AU - Shangold, Gary A.

AU - Kongsamut, Sathapana

AU - Miller, Richard J.

N1 - Funding Information: The authors would like to thank Ellsabeth Cornelison for excellent technical asslstance and Della Akres and Joyce Bonner for preparation of the manuscrlpt. This study was supported by NIH grants DA-02121 and DA-02575 to R.J.M. and a grant from The Mothers' Aid Research Fund of Chlcago Lylng-ln Hospital to G.A.S.

PY - 1985/6/10

Y1 - 1985/6/10

N2 - We have pharmacologically characterized voltage sensitive calcium channels (VSCCs) in GH3 cells, an anterior pituitary clonal cell line known to secrete prolactin and growth hormone. Raising the medium K+ concentration from 5 to 50 mM caused an immediate increase in net 45Ca2+ uptake which remained apparent over a 15 minute time course. 45Ca2+ uptake was maximally stimulated nearly 10-fold over basal levels. This K+-induced stimulation of Ca2+ uptake was not prevented by 10-5M tetrodotoxin or by replacing sodium with choline in the assay medium. Ca2+ uptake was, however, inhibited by several VSCC antagonists: nitrendipine, D-600, diltiazem and Cd2+. Further, the novel dihydropyridine VSCC agonists, BAY K8644 and CGP 28392, enhanced 50 mM K+-stimulated 45Ca2+ uptake and these effects were blocked by nitrendipine.

AB - We have pharmacologically characterized voltage sensitive calcium channels (VSCCs) in GH3 cells, an anterior pituitary clonal cell line known to secrete prolactin and growth hormone. Raising the medium K+ concentration from 5 to 50 mM caused an immediate increase in net 45Ca2+ uptake which remained apparent over a 15 minute time course. 45Ca2+ uptake was maximally stimulated nearly 10-fold over basal levels. This K+-induced stimulation of Ca2+ uptake was not prevented by 10-5M tetrodotoxin or by replacing sodium with choline in the assay medium. Ca2+ uptake was, however, inhibited by several VSCC antagonists: nitrendipine, D-600, diltiazem and Cd2+. Further, the novel dihydropyridine VSCC agonists, BAY K8644 and CGP 28392, enhanced 50 mM K+-stimulated 45Ca2+ uptake and these effects were blocked by nitrendipine.

UR - http://www.scopus.com/inward/record.url?scp=0021846845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021846845&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(85)90331-5

DO - 10.1016/0024-3205(85)90331-5

M3 - Article

C2 - 2582224

AN - SCOPUS:0021846845

SN - 0024-3205

VL - 36

SP - 2209

EP - 2215

JO - Life Sciences

JF - Life Sciences

IS - 23

ER -

Characterization of voltage-sensitive calcium channels in a clonal pituitary cell line

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this