Charged residues render pro-OmpA potential dependent for initiation of membrane translocation

Bruce Geller, Heng Yi Zhu, Shiyuan Cheng, Andreas Kuhn, Ross E. Dalbey

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

We have examined the effects of positively and negatively charged residues on the translocation of outer membrane protein A precursor (pro-OmpA) across the bacterial inner membrane. Pro-OmpA does not translocate across the membrane when 2 positively charged residues are inserted immediately after the leader peptide, whereas it does insert when 2 neutral or negatively charged residues are introduced. Using a cell-free translocation system, we show that the membrane potential stimulated the rate of initial insertion of pro-OmpA with negatively charged residues, inhibited pro-OmpA with positively charged residues, and had no effect on neutral pro-OmpA. Thus, acidic residues render pro-OmpA potential-dependent for loop formation, which then initiates the translocation process.

Original languageEnglish (US)
Pages (from-to)9442-9447
Number of pages6
JournalJournal of Biological Chemistry
Volume268
Issue number13
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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