CHD6 promotes broad nucleosome eviction for transcriptional activation in prostate cancer cells

Dongyu Zhao, Min Zhang, Shaodong Huang, Qi Liu, Sen Zhu, Yanqiang Li, Weihua Jiang, Daniel L. Kiss, Qi Cao*, Lili Zhang*, Kaifu Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Despite being a member of the chromodomain helicase DNA-binding protein family, little is known about the exact role of CHD6 in chromatin remodeling or cancer disease. Here we show that CHD6 binds to chromatin to promote broad nucleosome eviction for transcriptional activation of many cancer pathways. By integrating multiple patient cohorts for bioinformatics analysis of over a thousand prostate cancer datasets, we found CHD6 expression elevated in prostate cancer and associated with poor prognosis. Further comprehensive experiments demonstrated that CHD6 regulates oncogenicity of prostate cancer cells and tumor development in a murine xenograft model. ChIP-Seq for CHD6, along with MNase-Seq and RNA-Seq, revealed that CHD6 binds on chromatin to evict nucleosomes from promoters and gene bodies for transcriptional activation of oncogenic pathways. These results demonstrated a key function of CHD6 in evicting nucleosomes from chromatin for transcriptional activation of prostate cancer pathways.

Original languageEnglish (US)
Pages (from-to)12186-12201
Number of pages16
JournalNucleic acids research
Volume50
Issue number21
DOIs
StatePublished - Nov 28 2022

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'CHD6 promotes broad nucleosome eviction for transcriptional activation in prostate cancer cells'. Together they form a unique fingerprint.

Cite this