Chemiluminescence detection of gangliosides by thin-layer chromatography

S. L. Arnsmeier, A. S. Paller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We describe a method for the detection of gangliosides on thin-layer chromatography plates using enhanced chemiluminescence. In contrast to previously published colorimetric techniques, the use of chemiluminescence to detect antibody binding to gangliosides has increased sensitivity and simplicity. In addition, the use of chemiluminescence lacks the hazards of radioactivity, and produces a permanent record.

Original languageEnglish (US)
Pages (from-to)911-915
Number of pages5
JournalJournal of lipid research
Volume36
Issue number4
StatePublished - 1995

Keywords

  • enhanced chemiluminescence
  • immuno staining

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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