@article{f08a63bf6bfa478db9ae561a4b7073a1,
title = "Chemistry of oligonucleotide-gold nanoparticle conjugates",
abstract = "Conjugates prepared by immobilizing thiol-terminated oligonucleotides onto gold nanoparticles form stable colloidal solutions in aqueous media. The oligonucleotides can serve as linkers to organize the gold particles reversibly into three dimensional assemblies, and the gold particles can function as colorimetric reporters for hybridization of the bound oligomers with target oligonucleotides in solution.",
keywords = "Gold, Hybridization, Nanoparticles, Non-radioactive detection, Oligonucleotide",
author = "Letsinger, {Robert L.} and Mirkin, {Chad A.} and Robert Elghanian and Mucic, {Robert C.} and Storhoff, {James J.}",
note = "Funding Information: it was pink191. We attribute the sharp breaks in the melting transition in these cases to a high degree of cooperativity in dissociating the duplex strands holding the nanoparticle assemblies together and to the fact that the assemblies can exhibit their characteristic spectra even after dissociation of a considerable fraction of the interparticle linkages. As a consequence of the sharp melting transition, the nanoparticle probes are highly selective in recognizing oligonucleotide sequences. Probes V and VI can readily distinguish between Target II and an oligonucleotide differing by a single nucleotide191. For a related system we found that a 24-mer target could be identified by the spot test even in presence of four other oligonucleotides, each differing by a single nucleotide substitution, addition, or omission110'. In summary, oligonucleotides bound through a terminal sulfur atom to gold nanoparticles readily hybridize with other oligonucleotides free in solution or anchored to other nanoparticles. These conjugates provide a means for organizing nanoparticles reversibly into assemblies and show promise as highly selective probes for oligonucleotide targets. Acknowledgments This work was supported by the Lurie Cancer Center of Northwestern University, the National Institute of General Medical Sciences (GM058356), ONR, AOR, and NSF.",
year = "1999",
doi = "10.1080/10426509908546255",
language = "English (US)",
volume = "144-146",
pages = "359--362",
journal = "Phosphorus, Sulfur and Silicon and the Related Elements",
issn = "1042-6507",
publisher = "Taylor and Francis Ltd.",
}