Chemoenzymatic approaches for streamlined detection of active site modifications on thiotemplate assembly lines using mass spectrometry

Keyphrases

• Mass Spectrometry 100%
• Active Sites 100%
• Assembly Line 100%
• Thiotemplate 100%
• Chemoenzymatic Approach 100%
• Coenzyme A 60%
• Nonribosomal Peptide Synthetase 40%
• Pyochelin 40%
• Biotin 20%
• Selective Detection 20%
• High Purity 20%
• Side Reactions 20%
• Irradiation 20%
• Digestion 20%
• Siderophore 20%
• Chromatographic Separation 20%
• Escherichia Coli 20%
• Synthetase 20%
• Proteolysis 20%
• Acyl Chain 20%
• Quickest Detection 20%
• Absorbance 20%
• Pyrene 20%
• Fourier Transform Mass Spectrometry 20%
• Cyanogen Bromide 20%
• Aminoethyl 20%
• Mass Shift 20%
• Chromophoric 20%
• Thiolation Domain 20%
• Carrier Peptide 20%
• PchE 20%
• BODIPY FL 20%

Biochemistry, Genetics and Molecular Biology

• Active Site 100%
• Mass Spectrometry 100%
• Serine 60%
• Coenzyme A 60%
• Nonribosomal Peptide 40%
• Protein Catabolism 20%
• Anabolism 20%
• Fourier Transform 20%
• Chromatography 20%
• Enzyme 20%
• Escherichia coli 20%
• Biotin 20%
• Siderophore 20%