TY - JOUR
T1 - Chiral cyclohexane 1,3-diones as inhibitors of mutant SOD1-dependent protein aggregation for the treatment of ALS
AU - Zhang, Yinan
AU - Benmohamed, Radhia
AU - Zhang, Wei
AU - Kim, Jinho
AU - Edgerly, Christina K.
AU - Zhu, Yaoqiu
AU - Morimoto, Richard I.
AU - Ferrante, Robert J.
AU - Kirsch, Donald R.
AU - Silverman, Richard B.
PY - 2012/7/12
Y1 - 2012/7/12
N2 - Cyclohexane 1,3-diones were identified as a class of molecules exhibiting a protective effect against mutant SOD1 induced toxicity in PC-12 cells, but an optimized analogue had little or no effect on life extension in the G93A SOD1 mouse model for amyotrophic lateral sclerosis (ALS). Additional testing showed that these compounds were inactive in neurons, and further analogue synthesis was carried out to identify compounds with neuronal activity. Starting from two racemic derivatives that were active in cortical neurons, two potent analogues (1b and 2b) were resolved, which were protective against mutant SOD1 induced toxicity in PC-12 cells. Both compounds were found to be active in cortical neurons and presented good ADME profiles in vitro. On the basis of these results, an ALS mouse trial with 1b was carried out, which showed slightly greater life extension than the FDA-approved ALS drug riluzole, thereby validating cyclohexane 1,3-diones as a novel therapeutic class for the treatment of ALS.
AB - Cyclohexane 1,3-diones were identified as a class of molecules exhibiting a protective effect against mutant SOD1 induced toxicity in PC-12 cells, but an optimized analogue had little or no effect on life extension in the G93A SOD1 mouse model for amyotrophic lateral sclerosis (ALS). Additional testing showed that these compounds were inactive in neurons, and further analogue synthesis was carried out to identify compounds with neuronal activity. Starting from two racemic derivatives that were active in cortical neurons, two potent analogues (1b and 2b) were resolved, which were protective against mutant SOD1 induced toxicity in PC-12 cells. Both compounds were found to be active in cortical neurons and presented good ADME profiles in vitro. On the basis of these results, an ALS mouse trial with 1b was carried out, which showed slightly greater life extension than the FDA-approved ALS drug riluzole, thereby validating cyclohexane 1,3-diones as a novel therapeutic class for the treatment of ALS.
KW - Cyclohexane 1,3-diones
KW - PC-12 cells
KW - amyotrophic lateral sclerosis (ALS)
KW - cortical neurons
KW - mutant SOD1
KW - protein aggregation
KW - superoxide dismutase 1 (SOD1)
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U2 - 10.1021/ml3000963
DO - 10.1021/ml3000963
M3 - Article
C2 - 22837812
AN - SCOPUS:84863850645
SN - 1948-5875
VL - 3
SP - 584
EP - 587
JO - ACS Medicinal Chemistry Letters
JF - ACS Medicinal Chemistry Letters
IS - 7
ER -