Chiral discrimination among aminotransferases: Inactivation by 4-amino-4,5-dihydrothiophenecarboxylic Acid

Bryan W. Lepore, Dali Liu, Ying Peng, Mengmeng Fu, Chad Yasuda, James M. Manning, Richard B. Silverman, Dagmar Ringe*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Mechanism-based inhibitors such as cycloserine and gabaculine can inactivate aminotransferases via reactions of the compounds with the pyridoxal phosphate cofactor forming an irreversible adduct. The reaction is chirally specific in that any one enzyme usually only recognizes one enantiomer of the inactivator. For instance, l-aspartate aminotransferase (l-AspAT) is inactivated by 4-amino-4,5-dihydro-2-thiophenecarboxylic acid (ADTA), however, only by the S-isomer. We have now shown that d-amino acid aminotransferase (d-a-AT) is irreversibly inactivated by the R-isomer of the same compound. The X-ray crystal structure (PDB code: 3LQS) of the inactivated enzyme shows that in the product the enzyme no longer makes a Schiff base linkage to the pyridoxal 5'-phosphate (PLP) cofactor, and instead the compound has formed a derivative of the cofactor. The adduct is similar to that formed between d-cycloserine and d-a-AT or alanine racemase (Ala-Rac) in that the thiophene ring of R-ADTA is intact and seems to be aromatic. The plane of the ring is rotated by nearly 90 with respect to the plane of the pyridine ring of the cofactor, in comparison with the enzyme inactivated by cycloserine. Based on the structure of the product, the mechanism of inactivation most probably involves a transamination followed by aromatization to form an aromatic thiophene ring.

Original languageEnglish (US)
Pages (from-to)3138-3147
Number of pages10
Issue number14
StatePublished - Apr 13 2010

ASJC Scopus subject areas

  • Biochemistry


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