@article{80ed469e634f4a3689f86d9219e305ff,
title = "Chiral Recognition of Lipid Bilayer Membranes by Supramolecular Assemblies of Peptide Amphiphiles",
abstract = "On the basis of the exclusive existence of homochirality in biomolecules and the well-known phenomenon of chiral recognition, it is obvious that chirality is a crucial factor in biological events. We report here that supramolecular assemblies of peptide amphiphiles interact with lipid bilayer membranes in a stereospecific manner. When negatively charged chiral phospholipid bilayer vesicles were subjected to the assemblies, we found that peptide amphiphiles with l-amino acids show stronger affinity for the liposomes compared to the ones with d-amino acids. To examine their biological functions, we tested the cytotoxicity of nanofibers against mammalian primary cells using human bone marrow mesenchymal stem cells and murine astroglial cells. We demonstrated that cell viability increased when d-amino acids were incorporated in the structure of peptide amphiphiles, which is consistent with our finding of their weaker interactions with lipid bilayer membranes.",
keywords = "chiral recognition, lipid bilayer membrane, mammalian primary cells, peptide amphiphile, supramolecular assembly",
author = "Kohei Sato and Wei Ji and Zaida {\'A}lvarez and Palmer, {Liam C.} and Stupp, {Samuel I.}",
note = "Funding Information: ZaidaAWeiJi{\':}lvarez: 0000-0001-5104-13880000-0002-1473-5222 Liam C. Palmer: 0000-0003-0804-1168 Samuel I. Stupp: 0000-0002-5491-7442 Present Address △K.S. is currently at Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan 226-8501. Author Contributions The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. Funding This work was supported by the Center for Bio-Inspired Energy Sciences (CBES), an Energy Frontiers Research Center (EFRC) funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Award DESC0000989, and by the Japan Society for the Promotion of Science (JSPS) through its “Grant-in-Aid for Research Activity Start-up” Grant 18H05971. Dr. Wei Ji is a postdoctoral fellow of the Research Foundation Flanders (12G2718N), and received a Travel Grant of Long Stay Abroad (V468915N) from the Research Foundation Flanders (FWO-Vlaanderen), and Junior Mobility Programme (JuMo) of KU Leuven (JUMO-15−0514). Dr. Z. {\'A}lvarez has received postdoctoral support from the Beatriu de Pino{\'s} Fellowship 2014 BP-A 00007 (Ageǹcia de Gesti{\'o} d{\textquoteright}Ajust Universitaris i de Recerca, (AGAUR), Spain), and by Grant PVA17_RF_0008 from the Paralyzed Veterans of America Research Foundation. Notes The authors declare no competing financial interest. Funding Information: We are grateful to the following core facilities at Northwestern University: Biological Imaging Facility and Keck Biophysics Facility for instrument use. This work made use of the EPIC facility of Northwestern University{\textquoteright}s NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF NNCI-1542205); the MRSEC program (NSF DMR-1121262) at the Materials Research Center; the International Institute for Nanotechnology (IIN); the Keck Foundation; and the State of Illinois, through the IIN. We acknowledge Dr. Reiner Bleher and Eric W. Roth (NUANCE/EPIC) for assistance with cryogenic TEM. This work made use of the IMSERC at Northwestern University, which has received support from the SHyNE Resource; the State of Illinois; and the IIN. Biological experiments were performed in the Analytical BioNanoTech-nology Core Facility and peptide synthesis was performed in the Peptide Synthesis Core Facility, both of the Simpson Querrey Institute at Northwestern University. The U.S. Army Research Office, the U.S. Army Medical Research and Materiel Command, and Northwestern University provided funding to develop both of these facilities, and ongoing support is received from the SHyNE Resource. Portions of this work were performed at the DuPont-Northwestern-Dow Collaborative Access Team (DND-CAT) located at Sector 5 of the Advanced Photon Source (APS). DND-CAT is supported by Northwestern University, E.I. DuPont de Nemours & Co., and The Dow Chemical Company. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract DE-AC02-06CH11357. Data was collected using an instrument funded by the National Science Foundation under Award 0960140. We acknowledge Dr. Steven Weigand for assistance with small-angle X-ray scattering (SAXS) measurements. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",
year = "2019",
month = jun,
day = "10",
doi = "10.1021/acsbiomaterials.9b00553",
language = "English (US)",
volume = "5",
pages = "2786--2792",
journal = "ACS Biomaterial Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "6",
}