Chloroplast Microsatellite Markers for Artocarpus (Moraceae) Developed from Transcriptome Sequences

Elliot M. Gardner*, Kristen M. Laricchia, Matthew Murphy, Diane Ragone, Brian E. Scheffler, Sheron Simpson, Evelyn W. Williams, Nyree J.C. Zerega

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Premise of the study: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. Methods and Results: Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy. Conclusions: These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit.

Original languageEnglish (US)
Article number1500049
JournalApplications in Plant Sciences
Volume3
Issue number9
DOIs
StatePublished - Sep 1 2015

Keywords

  • Artocarpus altilis
  • Artocarpus camansi
  • Artocarpus mariannensis
  • Artocarpus odoratissimus
  • Moraceae
  • breadfruit

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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