CHO cell responses to low oxygen: Regulation of oxygen consumption and sensitization to oxidative stress

Andy A. Lin, William M. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

We have investigated the regulation of oxygen consumption and modulation of glutathione levels in CHO‐K1 cells under oxygen‐limiting conditions. We report here suppression of oxygen consumption and alteration of the supply‐dependent relationship as a consequence of prolonged hypoxic or anoxic exposure. The suppression is characterized by an increase in the value of P o 2/50 (the oxygen tension at which oxygen consumption is half maximal). Under prolonged anoxia there is also a decrease in the cells' potential to use oxygen. Elevated glucose consumption under low oxygen conditions may contribute to the suppression in respiration. The glutathione concentration remains constant throughout hypoxic exposure but may decrease by as much as 40% under anoxia. The glutathione level in hypoxic and anoxic cells increases by two‐ and four‐fold, respectively, over that of the control cells when exposed to a cytotoxic level of oxygen (93%). This suggests that anoxic and hypoxic exposure sensitizes CHO cells to oxidative stress. © 1992 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)505-516
Number of pages12
JournalBiotechnology and Bioengineering
Volume40
Issue number4
DOIs
StatePublished - Aug 5 1992

Keywords

  • Chinese hamster ovary cells
  • anoxia
  • glutathione
  • hypoxia
  • oxidative stress
  • oxygen consumption supply‐dependent relationship
  • reoxygenation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'CHO cell responses to low oxygen: Regulation of oxygen consumption and sensitization to oxidative stress'. Together they form a unique fingerprint.

  • Cite this