Cholesterol-induced suppression of Kir2 channels is mediated by decoupling at the inter-subunit interfaces

Nicolas Barbera*, Sara T. Granados, Carlos Guillermo Vanoye, Tatiana V. Abramova, Danielle Kulbak, Sang Joon Ahn, Alfred L. George, Belinda S. Akpa*, Irena Levitan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Cholesterol is a major regulator of multiple types of ion channels. Although there is increasing information about cholesterol binding sites, the molecular mechanisms through which cholesterol binding alters channel function are virtually unknown. In this study, we used a combination of Martini coarse-grained simulations, a network theory-based analysis, and electrophysiology to determine the effect of cholesterol on the dynamic structure of the Kir2.2 channel. We found that increasing membrane cholesterol reduced the likelihood of contact between specific regions of the cytoplasmic and transmembrane domains of the channel, most prominently at the subunit-subunit interfaces of the cytosolic domains. This decrease in contact was mediated by pairwise interactions of specific residues and correlated to the stoichiometry of cholesterol binding events. The predictions of the model were tested by site-directed mutagenesis of two identified residues—V265 and H222—and high throughput electrophysiology.

Original languageEnglish (US)
Article number104329
JournaliScience
Volume25
Issue number5
DOIs
StatePublished - May 20 2022

Keywords

  • Biophysics
  • Cellular physiology
  • Molecular biology

ASJC Scopus subject areas

  • General

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