Cholinergic Projections to the Substantia Nigra Pars Reticulata Inhibit Dopamine Modulation of Basal Ganglia through the M4 Muscarinic Receptor

Mark S. Moehle, Tristano Pancani, Nellie Byun, Samantha E. Yohn, George H. Wilson, Johnathan W. Dickerson, Daniel H. Remke, Zixiu Xiang, Colleen M. Niswender, Jürgen Wess, Carrie K. Jones, Craig W. Lindsley, Jerri M. Rook, P. Jeffrey Conn*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Cholinergic regulation of dopaminergic inputs into the striatum is critical for normal basal ganglia (BG) function. This regulation of BG function is thought to be primarily mediated by acetylcholine released from cholinergic interneurons (ChIs) acting locally in the striatum. We now report a combination of pharmacological, electrophysiological, optogenetic, chemogenetic, and functional magnetic resonance imaging studies suggesting extra-striatal cholinergic projections from the pedunculopontine nucleus to the substantia nigra pars reticulata (SNr) act on muscarinic acetylcholine receptor subtype 4 (M4) to oppose cAMP-dependent dopamine receptor subtype 1 (D1) signaling in presynaptic terminals of direct pathway striatal spiny projections neurons. This induces a tonic inhibition of transmission at direct pathway synapses and D1-mediated activation of motor activity. These studies provide important new insights into the unique role of M4 in regulating BG function and challenge the prevailing hypothesis of the centrality of striatal ChIs in opposing dopamine regulation of BG output. Moehle et al. show activation of M4 can induce inhibition of D1 signaling. Interestingly, this mechanism occurs on direct pathway terminals in the SNr from acetylcholine released by hindbrain cholinergic projections, expanding the model of cholinergic regulation of the BG.

Original languageEnglish (US)
Pages (from-to)1358-1372.e4
JournalNeuron
Volume96
Issue number6
DOIs
StatePublished - Dec 20 2017

ASJC Scopus subject areas

  • Neuroscience(all)

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