Chromatin immunoprecipitation

Grant D. Barish, Rajenda K. Tangirala

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Recent studies have elucidated molecular mechanisms underlying the transcriptional control of metabolism in complex metabolic disorders such as metabolic syndrome and atherosclerosis. Chromatin immunoprecipitation (ChIP) is an important technique to study protein-DNA interactions in vivo. Chemical cross-linking of DNA and its associated proteins, followed by chromatin shearing, immunoprecipitation of a protein of interest, DNA isolation, and PCR interrogation, can identify specific interactions between protein and DNA or sites of histone epigenetic alteration. Transcription factors and epigenetic modifications are key determinants of transcription. Accordingly, ChIP experiments can provide powerful mechanistic insights to understand gene expression.

Original languageEnglish (US)
Title of host publicationLipoproteins and Cardiovascular Disease
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc
Pages327-342
Number of pages16
ISBN (Print)9781603273688
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1027
ISSN (Print)1064-3745

Keywords

  • Cardiovascular disease
  • ChIP
  • Chromatin
  • Immunoprecipitation
  • Protein-DNA interaction
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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