Chromatin reconstruction during mouse terminal erythropoiesis

Honghao Bi, Ye Hou, Juan Wang, Zongjun Xia, Dongmei Wang, Yijie Liu, Haiyan Bao, Xu Han, Kehan Ren, Ermin Li, Feng Yue*, Peng Ji*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Mammalian terminal erythropoiesis involves chromatin and nuclear condensation followed by enucleation. Late-stage erythroblasts undergo caspase-mediated nuclear opening that is important for nuclear condensation through partial histone release. It remains unknown the dynamic changes of three-dimensional (3D) genomic organization during terminal erythropoiesis. Here, we used Hi-C to determine the chromatin structural change during primary mouse erythroblast terminal differentiation. We also performed RNA-sequencing and ATAC-sequencing under the same experimental setting to further reveal the genome accessibility and gene expression changes during this process. We found that late-stage terminal erythropoiesis involves global loss of topologically associating domains and establishment of inter-chromosomal interactions of the heterochromatin regions, which are associated with globally increased chromatin accessibility and upregulation of erythroid-related genes.

Original languageEnglish (US)
Article number105554
JournaliScience
Volume25
Issue number12
DOIs
StatePublished - Dec 22 2022

Funding

We thank Dr. Ching Man Wai’s technical support during this study. This work was supported by National Institute of Diabetes and Digestive and Kidney Disease ( NIDDK ) grant R01-DK124220 (P.J.), National Heart, Lung, and Blood Institute (NHLBI) grant R01-HL148012 (P.J.), and R01-HL150729 (P.J.). P.J. is a scholar of the Leukemia and Lymphoma Society . We thank Dr. Ching Man Wai's technical support during this study. This work was supported by National Institute of Diabetes and Digestive and Kidney Disease (NIDDK) grant R01-DK124220 (P.J.), National Heart, Lung, and Blood Institute (NHLBI) grant R01-HL148012 (P.J.), and R01-HL150729 (P.J.). P.J. is a scholar of the Leukemia and Lymphoma Society. H.B. Y.H. J.W. A.M. D.H. Y.L. H.B. X.H. K.R. and E.L. performed the experiments and interpreted data. Y.H. performed the Hi-C experiment. J.W. Z.X. and D.W. analyzed the Hi-C data. H.B. Y.L. F.Y. and P.J. designed the experiments, interpreted data, and edited the manuscript. H.B. and P.J. wrote the manuscript. The authors declare no competing interests.

Keywords

  • Cell biology
  • Structural biology

ASJC Scopus subject areas

  • General

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