Abstract
Mammalian terminal erythropoiesis involves chromatin and nuclear condensation followed by enucleation. Late-stage erythroblasts undergo caspase-mediated nuclear opening that is important for nuclear condensation through partial histone release. It remains unknown the dynamic changes of three-dimensional (3D) genomic organization during terminal erythropoiesis. Here, we used Hi-C to determine the chromatin structural change during primary mouse erythroblast terminal differentiation. We also performed RNA-sequencing and ATAC-sequencing under the same experimental setting to further reveal the genome accessibility and gene expression changes during this process. We found that late-stage terminal erythropoiesis involves global loss of topologically associating domains and establishment of inter-chromosomal interactions of the heterochromatin regions, which are associated with globally increased chromatin accessibility and upregulation of erythroid-related genes.
Original language | English (US) |
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Article number | 105554 |
Journal | iScience |
Volume | 25 |
Issue number | 12 |
DOIs | |
State | Published - Dec 22 2022 |
Funding
We thank Dr. Ching Man Wai’s technical support during this study. This work was supported by National Institute of Diabetes and Digestive and Kidney Disease ( NIDDK ) grant R01-DK124220 (P.J.), National Heart, Lung, and Blood Institute (NHLBI) grant R01-HL148012 (P.J.), and R01-HL150729 (P.J.). P.J. is a scholar of the Leukemia and Lymphoma Society . We thank Dr. Ching Man Wai's technical support during this study. This work was supported by National Institute of Diabetes and Digestive and Kidney Disease (NIDDK) grant R01-DK124220 (P.J.), National Heart, Lung, and Blood Institute (NHLBI) grant R01-HL148012 (P.J.), and R01-HL150729 (P.J.). P.J. is a scholar of the Leukemia and Lymphoma Society. H.B. Y.H. J.W. A.M. D.H. Y.L. H.B. X.H. K.R. and E.L. performed the experiments and interpreted data. Y.H. performed the Hi-C experiment. J.W. Z.X. and D.W. analyzed the Hi-C data. H.B. Y.L. F.Y. and P.J. designed the experiments, interpreted data, and edited the manuscript. H.B. and P.J. wrote the manuscript. The authors declare no competing interests.
Keywords
- Cell biology
- Structural biology
ASJC Scopus subject areas
- General