@article{b139e2d2a5f34fa39aca2e24faeb6cd9,
title = "Chromatin reconstruction during mouse terminal erythropoiesis",
abstract = "Mammalian terminal erythropoiesis involves chromatin and nuclear condensation followed by enucleation. Late-stage erythroblasts undergo caspase-mediated nuclear opening that is important for nuclear condensation through partial histone release. It remains unknown the dynamic changes of three-dimensional (3D) genomic organization during terminal erythropoiesis. Here, we used Hi-C to determine the chromatin structural change during primary mouse erythroblast terminal differentiation. We also performed RNA-sequencing and ATAC-sequencing under the same experimental setting to further reveal the genome accessibility and gene expression changes during this process. We found that late-stage terminal erythropoiesis involves global loss of topologically associating domains and establishment of inter-chromosomal interactions of the heterochromatin regions, which are associated with globally increased chromatin accessibility and upregulation of erythroid-related genes.",
keywords = "Cell biology, Structural biology",
author = "Honghao Bi and Ye Hou and Juan Wang and Zongjun Xia and Dongmei Wang and Yijie Liu and Haiyan Bao and Xu Han and Kehan Ren and Ermin Li and Feng Yue and Peng Ji",
note = "Funding Information: We thank Dr. Ching Man Wai{\textquoteright}s technical support during this study. This work was supported by National Institute of Diabetes and Digestive and Kidney Disease ( NIDDK ) grant R01-DK124220 (P.J.), National Heart, Lung, and Blood Institute (NHLBI) grant R01-HL148012 (P.J.), and R01-HL150729 (P.J.). P.J. is a scholar of the Leukemia and Lymphoma Society . Funding Information: We thank Dr. Ching Man Wai's technical support during this study. This work was supported by National Institute of Diabetes and Digestive and Kidney Disease (NIDDK) grant R01-DK124220 (P.J.), National Heart, Lung, and Blood Institute (NHLBI) grant R01-HL148012 (P.J.), and R01-HL150729 (P.J.). P.J. is a scholar of the Leukemia and Lymphoma Society. H.B. Y.H. J.W. A.M. D.H. Y.L. H.B. X.H. K.R. and E.L. performed the experiments and interpreted data. Y.H. performed the Hi-C experiment. J.W. Z.X. and D.W. analyzed the Hi-C data. H.B. Y.L. F.Y. and P.J. designed the experiments, interpreted data, and edited the manuscript. H.B. and P.J. wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = dec,
day = "22",
doi = "10.1016/j.isci.2022.105554",
language = "English (US)",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "12",
}