Chromatin reconstruction during mouse terminal erythropoiesis

Honghao Bi, Ye Hou, Juan Wang, Zongjun Xia, Dongmei Wang, Yijie Liu, Haiyan Bao, Xu Han, Kehan Ren, Ermin Li, Feng Yue*, Peng Ji*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Mammalian terminal erythropoiesis involves chromatin and nuclear condensation followed by enucleation. Late-stage erythroblasts undergo caspase-mediated nuclear opening that is important for nuclear condensation through partial histone release. It remains unknown the dynamic changes of three-dimensional (3D) genomic organization during terminal erythropoiesis. Here, we used Hi-C to determine the chromatin structural change during primary mouse erythroblast terminal differentiation. We also performed RNA-sequencing and ATAC-sequencing under the same experimental setting to further reveal the genome accessibility and gene expression changes during this process. We found that late-stage terminal erythropoiesis involves global loss of topologically associating domains and establishment of inter-chromosomal interactions of the heterochromatin regions, which are associated with globally increased chromatin accessibility and upregulation of erythroid-related genes.

Original languageEnglish (US)
Article number105554
Issue number12
StatePublished - Dec 22 2022


  • Cell biology
  • Structural biology

ASJC Scopus subject areas

  • General


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