Chromatographic separation of the C(1), C(1a), and C(2) components of gentamicin and the assessment of their individual binding to serum proteins

William C. Griffiths*, James F. Belliveau, Charlene L. Bensted, Steven G Calabresi, Israel Diamond

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

[3H]gentamicin and [14C]gentamicin samples were purified by Sephadex column chromatography and separated by an HPLC technique into the three major, medicinally active gentamicin components. These separated components were used in equilibrium dialysis studies to determine their percent binding to serum proteins. The bindings of the components were inversely related to concentrations of ionized calcium and magnesium. When dialyzed against a buffer containing physiological concentrations of the divalent cations, the binding of the C(1) component was 2.2 ± 1.0%, the binding of the C(1a) component was 1.2 ± 1.9%, and the binding of the C(2) component was 5.0 ± 2.0%. The percent bindings are not identical and, due to their low values, probably have negligible clinical significance. The radioactive composition and purity of the 3H‐ and 14C‐labeled gentamicin samples differed and these may be important factors in the variance of reported gentamicin bindings.

Original languageEnglish (US)
Pages (from-to)1836-1837
Number of pages2
JournalJournal of Pharmaceutical Sciences
Volume73
Issue number12
DOIs
StatePublished - Dec 1984

Keywords

  • Gentamicin—component separation serum protein binding, radiolabels, HPLC
  • Serum protein binding—radiolabeled gentamicin, component separation, HPLC

ASJC Scopus subject areas

  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Chromatographic separation of the C(1), C(1a), and C(2) components of gentamicin and the assessment of their individual binding to serum proteins'. Together they form a unique fingerprint.

Cite this