Abstract
Objective: To determine if idiopathic infertile men having oligozoospermia, asthenozoospermia, or teratozoospermia have an elevated risk of transmitting chromosomal abnormalities to their offspring. Design: Sperm chromosomal complements from five somatically normal infertile men were assayed using the human sperm-hamster oocyte fusion system and the disomy frequencies for chromosomes 1, 12, and the sex chromosomes were determined using fluorescence in situ hybridization. Setting: Infertile men and normal donors were volunteers in an academic research environment. Patients: Five men with the appropriate semen characteristics were recruited among patients concerned about their infertility. Interventions: Sperm fused with hamster oocytes resulting in sperm chromosomes or the sperm nuclei were prepared for fluorescence in situ hybridization. Main Outcome Measure: Structural and numerical abnormalities assessed by sperm karyotypes and the disomy frequency determined by fluorescence in situ hybridization analysis. Results: The infertile men showed increased frequencies of numerical abnormalities and total abnormalities as determined by sperm karyotyping. Analysis of sperm nuclei by fluorescence in situ hybridization indicated a significant increase in the frequency of disomy for chromosome 1 and XY disomy. Conclusions: Sperm from infertile men may contain an increased frequency of chromosomal abnormalities.
Original language | English (US) |
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Pages (from-to) | 811-817 |
Number of pages | 7 |
Journal | Fertility and Sterility |
Volume | 64 |
Issue number | 4 |
DOIs | |
State | Published - 1995 |
Keywords
- Male infertility
- fluorescence in situ hybridization
- sperm chromosomes
- sperm karyotypes
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine