Chromosome 9 Deletion Mapping Reveals Interferon a and Interferon ²-1 Gene Deletions in Human Glial Tumors

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Abstract

We have applied restriction fragment length polymorphism analysis to a 30-member panel of primary glioma DNAs, which had been previously examined for loss of genetic information (C. D. James, E. Carlbom, J. P. Dumanski, M. Hansen, M. Nordenskjold, V. P. Collins, and W. K. Cavenee, Cancer Res., 48:5546-5551, 1988), to determine the frequency and sublocalization of loss of genetic information from chromosome 9. We have also utilized scanning densitometry for dosage determination of the 9p-localized Interferon a and Interferon ß-\genes among these same tumors. Our results reveal the following: (a) for those cases in which loss has occurred, the region of common loss lies on the short (p) arm of the chromosome; (/») loss of genetic information from the short arm of chromosome 9 occurs frequently in glial tumors of intermediate (anaplastic, grade III) and high (glioblastoma, grade IV) histolã³gica!malig nancy (10 of 20 cases) but not in tumors of low (grade II) histolã³gica! malignancy (0 of 10 cases); (c) tumors with 9p deletions are hemi- or nullizygous for interferon, J-1 and the Interferon a gene cluster; (d) cases of interferon nullizygosity occur exclusively among tumors of highest histolã³gica!malignancy (glioblastoma). These data, especially the determination of a region of nullizygosity, suggest proximity to or residence within a gene(s) whose function(s) is (are) critical to the suppression of the malignant evolution of glial tumors.

Original languageEnglish (US)
Pages (from-to)1684-1688
Number of pages5
JournalCancer Research
Volume51
Issue number6
StatePublished - Mar 1991

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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