Chromosome analysis of spermatozoa extracted from testes of men with non-obstructive azoospermia (multiple letters)

Renée H. Martin, Calvin Greene, Alfred Rademaker, Leona Barclay, Evelyn Ko, Judy Chernos

Research output: Contribution to journalLetter

1 Scopus citations

Abstract

Infertile men with azoospermia now have the possibility of fathering children by testicular sperm extraction combined with intracytoplasmic sperm injection. However, there are concerns about the risk of chromosomal abnormalities in their spermatozoa. We have studied aneuploidy frequencies for chromosomes 13, 21, X and Y by multicolour fluorescence in-situ hybridization (FISH) in testicular spermatozoa extracted from three men with non-obstructive azoospermia. The men were 34–37 years of age and had normal follicle-stimulating hormone (FSH) concentrations and normal 46,XY somatic karyotypes. A total of 3324 spermatozoa was analysed. The infertile patients had an elevated frequency of disomy for chromosomes 13, 21, XY disomy compared to controls but none of these reached statistical significance. Also there was no significant difference in the sex ratio or the frequency of diploidy in azoospermic patients compared to normal control donors. This first report on chromosomal aneuploidy in spermatozoa extracted from testes of patients with non-obstructive azoospermia suggests that some azoospermic men do not have a substantially increased risk of chromosomally abnormal spermatozoa.

Original languageEnglish (US)
Pages (from-to)2685-2686
Number of pages2
JournalHuman Reproduction
Volume15
Issue number12
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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