Chromosome anomalies detected by interphase fluorescence in situ hybridization: Correlation with significant biological features of B-cell chronic lymphocytic leukaemia

Gordon W. Dewald*, Stephanie R. Brockman, Sarah F. Paternoster, Nancy D. Bone, Judith R. O'Fallon, Cristine Allmer, Charles D. James, Diane F. Jelinek, Renee C. Tschumper, Curtis A. Hanson, Rajiv K. Pruthi, Thomas E. Witzig, Timothy G. Call, Neil E. Kay

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

197 Scopus citations

Abstract

Fluorescence in situ hybridization (FISH) was used to detect 6q-, 11q-, +12, 13q-, 17p- and translocations involving 14q32 in interphase nuclei from blood and/or bone marrow from 113 patients with B-cell chronic lymphocytic leukaemia (B-CLL). A total of 87 patients (77%) had a FISH anomaly: 13q- × 1 was most frequent (64%) followed by 13q- × 2 (28%), +12 (25%), 11q-(15%), 17p- (8%) and 6q- (0%). FISH results for blood and bone marrow cells in 38 patients were similar. Purified CD5+/CD19+ cells from blood were studied in eight patients and results indicate that in some patients not all B cells have FISH anomalies. We used a defined set of hierarchical FISH risk categories to compare FISH results by stable versus progressive disease, age, sex, Rai stage, CD38+ expression and IgVH mutational status. Significant differences in FISH risk distributions were associated with Rai stage, disease status and CD38+, but not by age, sex or IgVH mutational status. To look for baseline factors associated with high-risk disease, multivariate analysis of age, sex, Rai stage, CD38+ and disease status versus FISH risk category was performed. Importantly, only CD38+ was significantly associated with high-risk FISH categories (+12, 11q- and 17p-) after adjustment for the effects of other variables.

Original languageEnglish (US)
Pages (from-to)287-295
Number of pages9
JournalBritish Journal of Haematology
Volume121
Issue number2
DOIs
StatePublished - Apr 2003

Keywords

  • B-CLL
  • CD38 expression
  • FISH
  • IgV mutations
  • Rai stage

ASJC Scopus subject areas

  • Hematology

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