Chromosome cohesion decreases in human eggs with advanced maternal age

Francesca E. Duncan, Jessica E. Hornick, Michael A. Lampson, Richard M. Schultz, Lonnie D. Shea, Teresa K. Woodruff*

*Corresponding author for this work

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Aneuploidy in human eggs increases with maternal age and can result in infertility, miscarriages, and birth defects. The molecular mechanisms leading to aneuploidy, however, are largely unknown especially in the human where eggs are exceedingly rare and precious. We obtained human eggs from subjects ranging from 16.4 to 49.7years old following in vitro maturation of oocyte-cumulus complexes isolated directly from surgically removed ovarian tissue. A subset of these eggs was used to investigate how age-associated aneuploidy occurs in the human. The inter-kinetochore distance between sister chromatids increased significantly with maternal age, indicating weakened cohesion. Moreover, we observed unpaired sister chromatids from females of advanced age. We conclude that loss of cohesion with increasing maternal age likely contributes to the well-documented increased incidence of aneuploidy.

Original languageEnglish (US)
Pages (from-to)1121-1124
Number of pages4
JournalAging Cell
Volume11
Issue number6
DOIs
StatePublished - Dec 1 2012

Keywords

  • Aneuploidy
  • Cohesion
  • Egg
  • Human
  • Maternal age
  • Meiosis

ASJC Scopus subject areas

  • Aging
  • Cell Biology

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