Chromosome conformation capture reveals two elements that interact with the PTBP3 (ROD1) transcription start site

Marta Kubiak, Anna Jurek, Katarzyna Kamińska, Janusz Kowalewski, Sui Huang, Marzena Anna Lewandowska*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The long-range control of gene expression is facilitated by chromatin looping and can be detected using chromosome conformation capture—3C. Here we focus on the chromatin architecture of the PTBP3 (Polypyrimidine tract binding protein 3) locus to evaluate its potential role in regulating expression of the gene. PTBP3 expression in prostate cancer cell lines is found significantly higher compared to skin fibroblasts using real-time PCR (p < 0.05) and digital droplet PCR (p < 0.01). Exploration of the chromatin spatial architecture of a nearly 200-kb fragment of chromosome 9 encompassing the PTBP3 gene identified two elements located 63 kb upstream and 48 kb downstream of PTBP3, which looped specifically to the PTBP3 promoter. These elements contain histone acetylation patterns characteristic of open chromatin regions with active enhancers. Our results reveal for the first time that long-range chromatin interactions between the −63 kb and +48 kb loci and the PTBP3 promoter regulate the expression of this gene in prostate cancer cells. These interactions support an open chromatin form for the PTBP3 locus in cancer cells and the three-dimensional structural model proposed in this paper.

Original languageEnglish (US)
Article number242
JournalInternational journal of molecular sciences
Issue number2
StatePublished - 2019


  • Cancer epigenetic
  • Chromatin conformation
  • Gene expression regulation
  • PTBP3
  • Prostate cancer
  • ROD1 (Regulator Of Differentiation 1)

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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