Chromosome organization of the protozoan Trypanosoma brucei

Keith Gottesdiener, Jaime Garciá-Ãnoveros, Mary Gwo Shu Lee, Lex H T Van der Ploeg

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The genome of the protozoan Trypanosoma brucei is known to be diploid. Karyotype analysis has, however, failed to identify homologous chromosomes. Having refined the technique for separating trypanosome chromosomes (L.H.T. Van Der Ploeg, C.L. Smith, R.I. Polvere, and K. Gottesdiener, Nucleic Acids Res. 17: 3217-3227, 1989), we can now provide evidence for the presence of homologous chromosomes. By determining the chromosomal location of different genetic markers, most of the chromosomes (14, excluding the minichromosomes), could be organized into seven chromosome pairs. In most instances, the putative homologs of a pair differed in size by about 20%. Restriction enzyme analysis of chromosome-sized DNA showed that these chromosome pairs contained large stretches of homologous DNA sequences. From these data, we infer that the chromosome pairs represent homologs. The identification of homologous chromosomes gives valuable insight into the organization of the trypanosome genome, will facilitate the genetic analysis of T. brucei, and suggests the presence of haploid gametes.

Original languageEnglish (US)
Pages (from-to)6079-6083
Number of pages5
JournalMolecular and Cellular Biology
Volume10
Issue number11
DOIs
StatePublished - Jan 1 1990

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Trypanosoma brucei brucei
Chromosomes
Trypanosomiasis
Protozoan Genome
Chromosomes, Human, Pair 14
Restriction Mapping
Haploidy
Sequence Homology
Diploidy
Karyotype
Genetic Markers
Germ Cells
Nucleic Acids
Genome
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Gottesdiener, Keith ; Garciá-Ãnoveros, Jaime ; Lee, Mary Gwo Shu ; Van der Ploeg, Lex H T. / Chromosome organization of the protozoan Trypanosoma brucei. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 11. pp. 6079-6083.
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Chromosome organization of the protozoan Trypanosoma brucei. / Gottesdiener, Keith; Garciá-Ãnoveros, Jaime; Lee, Mary Gwo Shu; Van der Ploeg, Lex H T.

In: Molecular and Cellular Biology, Vol. 10, No. 11, 01.01.1990, p. 6079-6083.

Research output: Contribution to journalArticle

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