Chronic lymphocytic leukemia FISH panel: Impact on diagnosis

Beverly P. Nelson*, Rohit Gupta, Gordon W. Dewald, Sarah F. Paternoster, Steven T. Rosen, Lo Ann C. Peterson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Interphase fluorescence in situ hybridization (FISH) is an alternative to conventional chromosome analysis of chronic lymphocytic leukemia (CLE) cells. We analyzed 172 samples from 136 possible CLL cases using a FISH panel. Reflex testing with probes to CCND1, BCL2, BCL3, BCL11A, c-MYC, MALT1, and a break-apart immunoglobulin heavy chain (IGH) probe was done if more than 2 signals for 14q32 occurred. For 111 cases, there were sufficient data for analysis. Of 111 cases, 81 (72.9%) had 1 or more genetic abnormalities. The most frequent abnormality was 13q-, followed by trisomy 12, 11q-, and 17p-. In 13 cases, there were IGH abnormalities. Two cases with CCND1/IGH fusion were reclassified as mantle cell lymphoma. Four CLL cases had IGH fusion with BCL2, BCL3 (2 cases), and BCL11A; no fusion partner was detected in 7 cases. Morphologic features were atypical for CLL in 2 cases with IGH fusion (BCL11A and BCL3). The FISH CLL panel is useful to identify prognostic aberrations and to clarify diagnosis in cases with unusual morphologic features.

Original languageEnglish (US)
Pages (from-to)323-332
Number of pages10
JournalAmerican journal of clinical pathology
Volume128
Issue number2
DOIs
StatePublished - Aug 2007

Keywords

  • 14q32 yranslocation
  • Chronic lymphocytic leukemia
  • FISH
  • Fluorescence in situ hybridization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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