Chronic neuropathic pain-like behavior correlates with IL-1β expression and disrupts cytokine interactions in the hippocampus

Adriana Del Rey*, Hau Jie Yau, Anke Randolf, Maria V. Centeno, Johannes Wildmann, Marco Martina, Hugo O. Besedovsky, Apkar Apkarian

*Corresponding author for this work

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We have proposed that neuropathic pain engages emotional learning, suggesting the involvement of the hippocampus. Because cytokines in the periphery contribute to induction and maintenance of neuropathic pain but might also participate centrally, we used 2 neuropathic pain models, chronic constriction injury (CCI) and spared nerve injury (SNI), to investigate the temporal profile of hippocampal cytokine gene expression in 2 rat strains that show different postinjury behavioral threshold sensitivities. SNI induced long-lasting allodynia in both strains, while CCI induced allodynia with time-dependent recovery in Sprague Dawley (SD) and no allodynia in Wistar Kyoto (WK) rats. In WK rats, only SNI induced sustained upregulation of hippocampal interleukin (IL)-1β, while IL-6 expression was transiently increased and no significant changes in IL-1ra expression were detected. Conversely, in SD rats, SNI resulted in sustained and robust increased hippocampal IL-1β expression, which was only transient in rats with CCI. In this strain, IL-6 expression was not affected in any of the 2 injury models and IL-1ra expression was significantly increased in rats with SNI or CCI at late phases. We found that the degree and development of neuropathic pain depend on the specific nerve injury model and rat strain; that hippocampal IL-1β mRNA levels correlate with neuropathic pain behavior; that, in contrast to sham-operated animals, there are no correlations between hippocampal IL-1β and IL-1ra or IL-6 in neuropathic rats; and that alterations in cytokine expression are restricted to the hippocampus contralateral to the injury side, again implying that the observed changes reflect nociception.

Original languageEnglish (US)
Pages (from-to)2827-2835
Number of pages9
JournalPain
Volume152
Issue number12
DOIs
StatePublished - Dec 1 2011

Fingerprint

Neuralgia
Interleukin-1
Chronic Pain
Hippocampus
Cytokines
Wounds and Injuries
Constriction
Interleukins
Hyperalgesia
Interleukin-6
Inbred WKY Rats
Nociception
Sprague Dawley Rats
Up-Regulation
Maintenance
Learning
Gene Expression

Keywords

  • Cytokines
  • Hippocampus
  • IL-1
  • IL-1ra
  • Lateralization
  • Neuropathic pain

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Del Rey, Adriana ; Yau, Hau Jie ; Randolf, Anke ; Centeno, Maria V. ; Wildmann, Johannes ; Martina, Marco ; Besedovsky, Hugo O. ; Apkarian, Apkar. / Chronic neuropathic pain-like behavior correlates with IL-1β expression and disrupts cytokine interactions in the hippocampus. In: Pain. 2011 ; Vol. 152, No. 12. pp. 2827-2835.
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Chronic neuropathic pain-like behavior correlates with IL-1β expression and disrupts cytokine interactions in the hippocampus. / Del Rey, Adriana; Yau, Hau Jie; Randolf, Anke; Centeno, Maria V.; Wildmann, Johannes; Martina, Marco; Besedovsky, Hugo O.; Apkarian, Apkar.

In: Pain, Vol. 152, No. 12, 01.12.2011, p. 2827-2835.

Research output: Contribution to journalArticle

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AU - Del Rey, Adriana

AU - Yau, Hau Jie

AU - Randolf, Anke

AU - Centeno, Maria V.

AU - Wildmann, Johannes

AU - Martina, Marco

AU - Besedovsky, Hugo O.

AU - Apkarian, Apkar

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N2 - We have proposed that neuropathic pain engages emotional learning, suggesting the involvement of the hippocampus. Because cytokines in the periphery contribute to induction and maintenance of neuropathic pain but might also participate centrally, we used 2 neuropathic pain models, chronic constriction injury (CCI) and spared nerve injury (SNI), to investigate the temporal profile of hippocampal cytokine gene expression in 2 rat strains that show different postinjury behavioral threshold sensitivities. SNI induced long-lasting allodynia in both strains, while CCI induced allodynia with time-dependent recovery in Sprague Dawley (SD) and no allodynia in Wistar Kyoto (WK) rats. In WK rats, only SNI induced sustained upregulation of hippocampal interleukin (IL)-1β, while IL-6 expression was transiently increased and no significant changes in IL-1ra expression were detected. Conversely, in SD rats, SNI resulted in sustained and robust increased hippocampal IL-1β expression, which was only transient in rats with CCI. In this strain, IL-6 expression was not affected in any of the 2 injury models and IL-1ra expression was significantly increased in rats with SNI or CCI at late phases. We found that the degree and development of neuropathic pain depend on the specific nerve injury model and rat strain; that hippocampal IL-1β mRNA levels correlate with neuropathic pain behavior; that, in contrast to sham-operated animals, there are no correlations between hippocampal IL-1β and IL-1ra or IL-6 in neuropathic rats; and that alterations in cytokine expression are restricted to the hippocampus contralateral to the injury side, again implying that the observed changes reflect nociception.

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