We examined the effect of chronic opiate treatment on the α2-adrenergic receptor binding of both the agonist [3H]clonidine and the antagonist [3H]yohimbine in the cerebral cortex and kidney of rats. In addition, we demonstrated the presence of an adrenergic receptor of the α2 type on the neurotumor cells line NCB20 and used this cell line as a model for studying the interaction between the opiate and α2-adrenergic systems. In all the three above systems, chronic opiate treatment did not modify the number or the affinity of α2-adrenergic receptors.
- NCB20 cell line
- Opiate dependence
- Rat cerebral cortex and kidney
- α-adrenergic receptors
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