Chronic opiate treatment does not modify α2-adrenergic receptors in rat cerebral cortex, kidney and in the neurotumor cell line NCB20

Lucia M. Vicentini; Richard J. Miller; Mark J. Robertson

doi:10.1016/0014-2999(83)90644-1

Chronic opiate treatment does not modify α₂-adrenergic receptors in rat cerebral cortex, kidney and in the neurotumor cell line NCB20

Lucia M. Vicentini, Richard J. Miller^*, Mark J. Robertson

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We examined the effect of chronic opiate treatment on the α₂-adrenergic receptor binding of both the agonist [³H]clonidine and the antagonist [³H]yohimbine in the cerebral cortex and kidney of rats. In addition, we demonstrated the presence of an adrenergic receptor of the α₂ type on the neurotumor cells line NCB20 and used this cell line as a model for studying the interaction between the opiate and α₂-adrenergic systems. In all the three above systems, chronic opiate treatment did not modify the number or the affinity of α₂-adrenergic receptors.

Original language	English (US)
Pages (from-to)	265-270
Number of pages	6
Journal	European Journal of Pharmacology
Volume	95
Issue number	3-4
DOIs	https://doi.org/10.1016/0014-2999(83)90644-1
State	Published - Nov 25 1983

Funding

This research was supported by PHS grant DA02121 R J.M. is an Alfred P Sloan Fellow. The authors thank Dr. David R Brown for helpful discussion.

Keywords

NCB20 cell line
Opiate dependence
Rat cerebral cortex and kidney
α-adrenergic receptors

ASJC Scopus subject areas

Pharmacology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0014-2999(83)90644-1

Cite this

@article{c38550b162c847388c45b6079a29c3a9,

title = "Chronic opiate treatment does not modify α2-adrenergic receptors in rat cerebral cortex, kidney and in the neurotumor cell line NCB20",

abstract = "We examined the effect of chronic opiate treatment on the α2-adrenergic receptor binding of both the agonist [3H]clonidine and the antagonist [3H]yohimbine in the cerebral cortex and kidney of rats. In addition, we demonstrated the presence of an adrenergic receptor of the α2 type on the neurotumor cells line NCB20 and used this cell line as a model for studying the interaction between the opiate and α2-adrenergic systems. In all the three above systems, chronic opiate treatment did not modify the number or the affinity of α2-adrenergic receptors.",

keywords = "NCB20 cell line, Opiate dependence, Rat cerebral cortex and kidney, α-adrenergic receptors",

author = "Vicentini, {Lucia M.} and Miller, {Richard J.} and Robertson, {Mark J.}",

note = "Funding Information: This research was supported by PHS grant DA02121 R J.M. is an Alfred P Sloan Fellow. The authors thank Dr. David R Brown for helpful discussion.",

year = "1983",

month = nov,

day = "25",

doi = "10.1016/0014-2999(83)90644-1",

language = "English (US)",

volume = "95",

pages = "265--270",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier B.V.",

number = "3-4",

}

TY - JOUR

T1 - Chronic opiate treatment does not modify α2-adrenergic receptors in rat cerebral cortex, kidney and in the neurotumor cell line NCB20

AU - Vicentini, Lucia M.

AU - Miller, Richard J.

AU - Robertson, Mark J.

N1 - Funding Information: This research was supported by PHS grant DA02121 R J.M. is an Alfred P Sloan Fellow. The authors thank Dr. David R Brown for helpful discussion.

PY - 1983/11/25

Y1 - 1983/11/25

N2 - We examined the effect of chronic opiate treatment on the α2-adrenergic receptor binding of both the agonist [3H]clonidine and the antagonist [3H]yohimbine in the cerebral cortex and kidney of rats. In addition, we demonstrated the presence of an adrenergic receptor of the α2 type on the neurotumor cells line NCB20 and used this cell line as a model for studying the interaction between the opiate and α2-adrenergic systems. In all the three above systems, chronic opiate treatment did not modify the number or the affinity of α2-adrenergic receptors.

AB - We examined the effect of chronic opiate treatment on the α2-adrenergic receptor binding of both the agonist [3H]clonidine and the antagonist [3H]yohimbine in the cerebral cortex and kidney of rats. In addition, we demonstrated the presence of an adrenergic receptor of the α2 type on the neurotumor cells line NCB20 and used this cell line as a model for studying the interaction between the opiate and α2-adrenergic systems. In all the three above systems, chronic opiate treatment did not modify the number or the affinity of α2-adrenergic receptors.

KW - NCB20 cell line

KW - Opiate dependence

KW - Rat cerebral cortex and kidney

KW - α-adrenergic receptors

UR - http://www.scopus.com/inward/record.url?scp=0021065191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021065191&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(83)90644-1

DO - 10.1016/0014-2999(83)90644-1

M3 - Article

C2 - 6317412

AN - SCOPUS:0021065191

SN - 0014-2999

VL - 95

SP - 265

EP - 270

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 3-4

ER -