Circadian Clock Interaction with HIF1α Mediates Oxygenic Metabolism and Anaerobic Glycolysis in Skeletal Muscle

Clara Bien, Daniel C. Levine, Jonathan Cedernaes, Akihiko Taguchi, Yumiko Kobayashi, Stacy J. Tsai, Nicolle A. Bonar, Maureen R. McNulty, Kathryn M Ramsey, Joseph Bass*

*Corresponding author for this work

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Circadian clocks are encoded by a transcription-translation feedback loop that aligns energetic processes with the solar cycle. We show that genetic disruption of the clock activator BMAL1 in skeletal myotubes and fibroblasts increased levels of the hypoxia-inducible factor 1α (HIF1α) under hypoxic conditions. Bmal1−/− myotubes displayed reduced anaerobic glycolysis, mitochondrial respiration with glycolytic fuel, and transcription of HIF1α targets Phd3, Vegfa, Mct4, Pk-m, and Ldha, whereas abrogation of the clock repressors CRY1/2 stabilized HIF1α in response to hypoxia. HIF1α bound directly to core clock gene promoters, and, when co-expressed with BMAL1, led to transactivation of PER2-LUC and HRE-LUC reporters. Further, genetic stabilization of HIF1α in Vhl−/− cells altered circadian transcription. Finally, induction of clock and HIF1α target genes in response to strenuous exercise varied according to the time of day in wild-type mice. Collectively, our results reveal bidirectional interactions between circadian and HIF pathways that influence metabolic adaptation to hypoxia.

Original languageEnglish (US)
Pages (from-to)86-92
Number of pages7
JournalCell Metabolism
Volume25
Issue number1
DOIs
StatePublished - Jan 10 2017

Fingerprint

Anaerobiosis
Hypoxia-Inducible Factor 1
Circadian Clocks
Glycolysis
Skeletal Muscle
Skeletal Muscle Fibers
Metabolic Networks and Pathways
Transcriptional Activation
Genes
Respiration
Fibroblasts

Keywords

  • BMAL1
  • HIF
  • circadian
  • clock
  • exercise
  • glycolysis
  • hypoxia
  • lactate
  • mitochondria
  • skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Bien, Clara ; Levine, Daniel C. ; Cedernaes, Jonathan ; Taguchi, Akihiko ; Kobayashi, Yumiko ; Tsai, Stacy J. ; Bonar, Nicolle A. ; McNulty, Maureen R. ; Ramsey, Kathryn M ; Bass, Joseph. / Circadian Clock Interaction with HIF1α Mediates Oxygenic Metabolism and Anaerobic Glycolysis in Skeletal Muscle. In: Cell Metabolism. 2017 ; Vol. 25, No. 1. pp. 86-92.
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Circadian Clock Interaction with HIF1α Mediates Oxygenic Metabolism and Anaerobic Glycolysis in Skeletal Muscle. / Bien, Clara; Levine, Daniel C.; Cedernaes, Jonathan; Taguchi, Akihiko; Kobayashi, Yumiko; Tsai, Stacy J.; Bonar, Nicolle A.; McNulty, Maureen R.; Ramsey, Kathryn M; Bass, Joseph.

In: Cell Metabolism, Vol. 25, No. 1, 10.01.2017, p. 86-92.

Research output: Contribution to journalArticle

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AU - Bass, Joseph

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