Circadian measurements of sirtuin biology

Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee Kyung Hong, Joseph Bass

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Many of our behavioral and physiological processes display daily oscillations that are under the control of the circadian clock. The core molecular clock network is present in both the brain and peripheral tissues and is composed of a complex series of interlocking transcriptional/translational feedback loops that oscillate with a periodicity of ~24 h. Recent evidence has implicated NAD+ biosynthesis and the sirtuin family of NAD +-dependent protein deacetylases as part of a novel feedback loop within the core clock network, findings which underscore the importance of taking circadian timing into consideration when designing and interpreting metabolic studies, particularly in regard to sirtuin biology. Thus, this chapter introduces both in vivo and in vitro circadian methods to analyze various sirtuin-related endpoints across the light-dark cycle and discusses the transcriptional, biochemical, and physiological outputs of the clock.

Original languageEnglish (US)
Title of host publicationSirtuins
Subtitle of host publicationMethods and Protocols
EditorsMatthew Hirschey
Pages285-302
Number of pages18
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1077
ISSN (Print)1064-3745

Keywords

  • Circadian
  • Clock
  • Constant darkness
  • Metabolism
  • NAD
  • Sirtuins
  • Synchronization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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