Circadian regulation of cellular physiology

Clara Bien, Kathryn M Ramsey, D. C. Levine, B. Marcheva, M. Perelis, Joseph Bass

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The circadian clock synchronizes behavioral and physiological processes on a daily basis in anticipation of the light-dark cycle. In mammals, molecular clocks are present in both the central pacemaker neurons and in nearly all peripheral tissues. Clock transcription factors in metabolic tissues coordinate metabolic fuel utilization and storage with alternating periods of feeding and fasting corresponding to the rest-activity cycle. In vitro and in vivo biochemical approaches have led to the discovery of mechanisms underlying the interplay between the molecular clock and the metabolic networks. For example, recent studies have demonstrated that the circadian clock controls rhythmic synthesis of the cofactor nicotinamide adenine dinucleotide (NAD+) and activity of NAD+-dependent sirtuin deacetylase enzymes to regulate mitochondrial function across the circadian cycle. In this chapter, we review current state-of-the-art methods to analyze circadian cycles in mitochondrial bioenergetics, glycolysis, and nucleotide metabolism in both cell-based and animal models.

Original languageEnglish (US)
Pages (from-to)165-184
Number of pages20
JournalMethods in Enzymology
Volume552
DOIs
StatePublished - Jan 1 2015

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Physiology
NAD
Clocks
Circadian Clocks
Physiological Phenomena
Activity Cycles
Photoperiod
Glycolysis
Metabolic Networks and Pathways
Energy Metabolism
Mammals
Fasting
Tissue
Transcription Factors
Nucleotides
Animal Models
Pacemakers
Neurons
Metabolism
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Bien, Clara ; Ramsey, Kathryn M ; Levine, D. C. ; Marcheva, B. ; Perelis, M. ; Bass, Joseph. / Circadian regulation of cellular physiology. In: Methods in Enzymology. 2015 ; Vol. 552. pp. 165-184.
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Circadian regulation of cellular physiology. / Bien, Clara; Ramsey, Kathryn M; Levine, D. C.; Marcheva, B.; Perelis, M.; Bass, Joseph.

In: Methods in Enzymology, Vol. 552, 01.01.2015, p. 165-184.

Research output: Contribution to journalArticle

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