Circular dichroism of double-helical oligoribonucleotides

Philip N. Borer; Olke C. Uhlenbeck; Barbara Dengler; Ignacio Tinoco

doi:10.1016/0022-2836(73)90208-8

Circular dichroism of double-helical oligoribonucleotides

Philip N. Borer^*, Olke C. Uhlenbeck, Barbara Dengler, Ignacio Tinoco

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The ultraviolet circular dichroism and absorption of 15 double-stranded helical oligoribonucleotides have been measured. These molecules of chain-length 6 to 12 contain all 10 possible nearest neighbors of Watson-Crick base pairs. They are thus good models for short double-stranded regions in RNA molecules. The contribution to the circular dichroism of each of the nearest neighbor base pairs has been obtained. The circular dichroism is found to be very sequence-dependent and may be useful in distinguishing possible secondary structures. However, the nearest neighbor approximation for circular dichroism fails to give a quantitative measure of the circular dichroism of double-strand regions.

Original language	English (US)
Pages (from-to)	759-771
Number of pages	13
Journal	Journal of Molecular Biology
Volume	80
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(73)90208-8
State	Published - Nov 15 1973

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(73)90208-8

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@article{0d51eeafaeea4dc88445e4f05f51c6b6,

title = "Circular dichroism of double-helical oligoribonucleotides",

abstract = "The ultraviolet circular dichroism and absorption of 15 double-stranded helical oligoribonucleotides have been measured. These molecules of chain-length 6 to 12 contain all 10 possible nearest neighbors of Watson-Crick base pairs. They are thus good models for short double-stranded regions in RNA molecules. The contribution to the circular dichroism of each of the nearest neighbor base pairs has been obtained. The circular dichroism is found to be very sequence-dependent and may be useful in distinguishing possible secondary structures. However, the nearest neighbor approximation for circular dichroism fails to give a quantitative measure of the circular dichroism of double-strand regions.",

author = "Borer, {Philip N.} and Uhlenbeck, {Olke C.} and Barbara Dengler and Ignacio Tinoco",

note = "Funding Information: This work was supported by grants from the National Institutes of Health (GM 10840 and GM 41393). We thank Mr David Koh for his assistance in preparing the compounds and Mr Cemal Kemal who determined the effect of deleting specific oligomer spectra on the c.d. contributions. Mr Kemal also looked for magnesium-dependent spectral changes.",

year = "1973",

month = nov,

day = "15",

doi = "10.1016/0022-2836(73)90208-8",

language = "English (US)",

volume = "80",

pages = "759--771",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

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T1 - Circular dichroism of double-helical oligoribonucleotides

AU - Borer, Philip N.

AU - Uhlenbeck, Olke C.

AU - Dengler, Barbara

AU - Tinoco, Ignacio

N1 - Funding Information: This work was supported by grants from the National Institutes of Health (GM 10840 and GM 41393). We thank Mr David Koh for his assistance in preparing the compounds and Mr Cemal Kemal who determined the effect of deleting specific oligomer spectra on the c.d. contributions. Mr Kemal also looked for magnesium-dependent spectral changes.

PY - 1973/11/15

Y1 - 1973/11/15

N2 - The ultraviolet circular dichroism and absorption of 15 double-stranded helical oligoribonucleotides have been measured. These molecules of chain-length 6 to 12 contain all 10 possible nearest neighbors of Watson-Crick base pairs. They are thus good models for short double-stranded regions in RNA molecules. The contribution to the circular dichroism of each of the nearest neighbor base pairs has been obtained. The circular dichroism is found to be very sequence-dependent and may be useful in distinguishing possible secondary structures. However, the nearest neighbor approximation for circular dichroism fails to give a quantitative measure of the circular dichroism of double-strand regions.

AB - The ultraviolet circular dichroism and absorption of 15 double-stranded helical oligoribonucleotides have been measured. These molecules of chain-length 6 to 12 contain all 10 possible nearest neighbors of Watson-Crick base pairs. They are thus good models for short double-stranded regions in RNA molecules. The contribution to the circular dichroism of each of the nearest neighbor base pairs has been obtained. The circular dichroism is found to be very sequence-dependent and may be useful in distinguishing possible secondary structures. However, the nearest neighbor approximation for circular dichroism fails to give a quantitative measure of the circular dichroism of double-strand regions.

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JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

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Circular dichroism of double-helical oligoribonucleotides

Abstract

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