Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5

Heather A. Pilch-Cooper, Scott F. Sieg, Thomas J. Hope, Ann Koons, Jean Michel Escola, Robin Offord, Ronald S. Veazey, Donald E. Mosier, Brian Clagett, Kathy Medvik, Julie K. Jadlowsky, Mark R. Chance, Janna G. Kiselar, James A. Hoxie, Ronald G. Collman, Nadeene E. Riddick, Valentina Mercanti, Oliver Hartley, Michael M. Lederman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


CC Chemokine Receptor 5 (CCR5) is an important mediator of chemotaxis and the primary coreceptor for HIV-1. A recent report by other researchers suggested that primary T cells harbor pools of intracellular CCR5. With the use of a series of complementary techniques to measure CCR5 expression (antibody labeling, Western blot, quantitative reverse transcription polymerase chain reaction), we established that intracellular pools of CCR5 do not exist and that the results obtained by the other researchers were false-positives that arose because of the generation of irrelevant binding sites for anti-CCR5 antibodies during fixation and permeabilization of cells.

Original languageEnglish (US)
Pages (from-to)1015-1019
Number of pages5
Issue number4
StatePublished - Jul 28 2011

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology


Dive into the research topics of 'Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5'. Together they form a unique fingerprint.

Cite this