Circulating microRNA signature for the diagnosis of very high-risk prostate cancer

Ali H. Alhasan, Alexander W. Scott, Jia J. Wu, Gang Feng, Joshua J. Meeks*, C. Shad Thaxton, Chad A. Mirkin

*Corresponding author for this work

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

We report the identification of a molecular signature using the Scano-miR profiling platform based on the differential expression of circulating microRNAs (miRNA, miR) in serum samples specific to patients with very high-risk (VHR) prostate cancer (PCa). Five miRNA PCa biomarkers (miR-200c, miR-605, miR-135a∗, miR-433, and miR-106a) were identified as useful for differentiating indolent and aggressive forms of PCa. All patients with VHR PCa in the study had elevated serum levels of miR-200c. Circulating miR-433, which was differentially expressed in patients with VHR versus low-risk (LR) forms of PCa, was not detectable by quantitative real-time PCR in samples from healthy volunteers. In blind studies, the five miRNA PCa biomarkers were able to differentiate patients with VHR PCas from those with LR forms as well as healthy individuals with at least 89% accuracy. Biological pathway analysis showed the predictive capability of these miRNA biomarkers for the diagnosis and prognosis of VHR aggressive PCa.

Original languageEnglish (US)
Pages (from-to)10655-10660
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number38
DOIs
StatePublished - Sep 20 2016

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Keywords

  • Biomarker
  • Prostate cancer
  • Scano-miR
  • Spherical nucleic acid
  • microRNA

ASJC Scopus subject areas

  • General

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