Circulating Tumor Cells with Epithelial–to–mesenchymal Transition Phenotypes Associated with Inferior Outcomes in Primary Breast Cancer

Michal Mego; Marian Karaba; Gabriel Minarik; Juraj Benca; Jurisova Silvia; Tatiana Sedlackova; Denisa Manasova; Katarina Kalavska; Daniel Pindak; Massimo Cristofanilli; James M. Reuben; Jozef Mardiak

doi:10.21873/anticanres.13290

Circulating Tumor Cells with Epithelial–to–mesenchymal Transition Phenotypes Associated with Inferior Outcomes in Primary Breast Cancer

Michal Mego^*, Marian Karaba, Gabriel Minarik, Juraj Benca, Jurisova Silvia, Tatiana Sedlackova, Denisa Manasova, Katarina Kalavska, Daniel Pindak, Massimo Cristofanilli, James M. Reuben, Jozef Mardiak

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Background/Aim: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. Patients and Methods: This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). Results: In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. Conclusion: CTCs with an EMT phenotype have a prognostic value in primary breast cancer.

Original language	English (US)
Pages (from-to)	1829-1837
Number of pages	9
Journal	Anticancer research
Volume	39
Issue number	4
DOIs	https://doi.org/10.21873/anticanres.13290
State	Published - Apr 2019

Keywords

Circulating tumor cells
Early breast cancer
Epithelial–to–mesenchymal transition

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/anticanres.13290

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Mego, M., Karaba, M., Minarik, G., Benca, J., Silvia, J., Sedlackova, T., Manasova, D., Kalavska, K., Pindak, D., Cristofanilli, M., Reuben, J. M., & Mardiak, J. (2019). Circulating Tumor Cells with Epithelial–to–mesenchymal Transition Phenotypes Associated with Inferior Outcomes in Primary Breast Cancer. Anticancer research, 39(4), 1829-1837. https://doi.org/10.21873/anticanres.13290

@article{e833afd2ee9b443381f176d3b6199207,

title = "Circulating Tumor Cells with Epithelial–to–mesenchymal Transition Phenotypes Associated with Inferior Outcomes in Primary Breast Cancer",

abstract = "Background/Aim: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. Patients and Methods: This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). Results: In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. Conclusion: CTCs with an EMT phenotype have a prognostic value in primary breast cancer.",

keywords = "Circulating tumor cells, Early breast cancer, Epithelial–to–mesenchymal transition",

author = "Michal Mego and Marian Karaba and Gabriel Minarik and Juraj Benca and Jurisova Silvia and Tatiana Sedlackova and Denisa Manasova and Katarina Kalavska and Daniel Pindak and Massimo Cristofanilli and Reuben, {James M.} and Jozef Mardiak",

note = "Funding Information: This publication is the result of the implementation of project no. APVV-16-0010 and APVV-14-0327, which were funded by the Slovak Research and Development Agency. Publisher Copyright: {\textcopyright} 2019 International Institute of Anticancer Research. All rights reserved.",

year = "2019",

month = apr,

doi = "10.21873/anticanres.13290",

language = "English (US)",

volume = "39",

pages = "1829--1837",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "4",

}

Mego, M, Karaba, M, Minarik, G, Benca, J, Silvia, J, Sedlackova, T, Manasova, D, Kalavska, K, Pindak, D, Cristofanilli, M, Reuben, JM & Mardiak, J 2019, 'Circulating Tumor Cells with Epithelial–to–mesenchymal Transition Phenotypes Associated with Inferior Outcomes in Primary Breast Cancer', Anticancer research, vol. 39, no. 4, pp. 1829-1837. https://doi.org/10.21873/anticanres.13290

TY - JOUR

T1 - Circulating Tumor Cells with Epithelial–to–mesenchymal Transition Phenotypes Associated with Inferior Outcomes in Primary Breast Cancer

AU - Mego, Michal

AU - Karaba, Marian

AU - Minarik, Gabriel

AU - Benca, Juraj

AU - Silvia, Jurisova

AU - Sedlackova, Tatiana

AU - Manasova, Denisa

AU - Kalavska, Katarina

AU - Pindak, Daniel

AU - Cristofanilli, Massimo

AU - Reuben, James M.

AU - Mardiak, Jozef

N1 - Funding Information: This publication is the result of the implementation of project no. APVV-16-0010 and APVV-14-0327, which were funded by the Slovak Research and Development Agency. Publisher Copyright: © 2019 International Institute of Anticancer Research. All rights reserved.

PY - 2019/4

Y1 - 2019/4

N2 - Background/Aim: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. Patients and Methods: This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). Results: In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. Conclusion: CTCs with an EMT phenotype have a prognostic value in primary breast cancer.

AB - Background/Aim: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. Patients and Methods: This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). Results: In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. Conclusion: CTCs with an EMT phenotype have a prognostic value in primary breast cancer.

KW - Circulating tumor cells

KW - Early breast cancer

KW - Epithelial–to–mesenchymal transition

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AN - SCOPUS:85064480384

SN - 0250-7005

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SP - 1829

EP - 1837

JO - Anticancer Research

JF - Anticancer Research

IS - 4

ER -

Circulating Tumor Cells with Epithelial–to–mesenchymal Transition Phenotypes Associated with Inferior Outcomes in Primary Breast Cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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