Circulation-independent differentiation pathway from extraembryonic mesoderm toward hematopoietic stem cells via hemogenic angioblasts

Yosuke Tanaka; Veronica Sanchez; Nozomu Takata; Tomomasa Yokomizo; Yojiro Yamanaka; Hiroshi Kataoka; Philipp S. Hoppe; Timm Schroeder; Shin Ichi Nishikawa

doi:10.1016/j.celrep.2014.05.055

Circulation-independent differentiation pathway from extraembryonic mesoderm toward hematopoietic stem cells via hemogenic angioblasts

Yosuke Tanaka^*, Veronica Sanchez, Nozomu Takata, Tomomasa Yokomizo, Yojiro Yamanaka, Hiroshi Kataoka, Philipp S. Hoppe, Timm Schroeder, Shin Ichi Nishikawa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

A large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1⁺ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) region and HSCs in the adult bone marrow. Here, we show that two Runx1⁺ populations subdivided by Gata1 expression exist in E7.5 embryos. The hemogenic endothelium and the HSCs are derived only from the Runx1⁺Gata1^- population. A subset of this population moves from the extra- to intraembryonic region during E7.5-E8.0, where it contributes to the hemogenic endothelium of the dorsal aorta (DA). Migration occurs before the heartbeat is initiated, and it is independent of circulation. This suggests a developmental trajectory from Runx1⁺ cells in the E7.5 extraembryonic region to definitive HSCs via the hemogenic endothelium.

Original language	English (US)
Pages (from-to)	31-39
Number of pages	9
Journal	Cell reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/j.celrep.2014.05.055
State	Published - Jul 10 2014
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2014.05.055

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title = "Circulation-independent differentiation pathway from extraembryonic mesoderm toward hematopoietic stem cells via hemogenic angioblasts",

abstract = "A large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1+ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) region and HSCs in the adult bone marrow. Here, we show that two Runx1+ populations subdivided by Gata1 expression exist in E7.5 embryos. The hemogenic endothelium and the HSCs are derived only from the Runx1+Gata1- population. A subset of this population moves from the extra- to intraembryonic region during E7.5-E8.0, where it contributes to the hemogenic endothelium of the dorsal aorta (DA). Migration occurs before the heartbeat is initiated, and it is independent of circulation. This suggests a developmental trajectory from Runx1+ cells in the E7.5 extraembryonic region to definitive HSCs via the hemogenic endothelium.",

author = "Yosuke Tanaka and Veronica Sanchez and Nozomu Takata and Tomomasa Yokomizo and Yojiro Yamanaka and Hiroshi Kataoka and Hoppe, {Philipp S.} and Timm Schroeder and Nishikawa, {Shin Ichi}",

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doi = "10.1016/j.celrep.2014.05.055",

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AU - Tanaka, Yosuke

AU - Sanchez, Veronica

AU - Takata, Nozomu

AU - Yokomizo, Tomomasa

AU - Yamanaka, Yojiro

AU - Kataoka, Hiroshi

AU - Hoppe, Philipp S.

AU - Schroeder, Timm

AU - Nishikawa, Shin Ichi

PY - 2014/7/10

Y1 - 2014/7/10

N2 - A large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1+ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) region and HSCs in the adult bone marrow. Here, we show that two Runx1+ populations subdivided by Gata1 expression exist in E7.5 embryos. The hemogenic endothelium and the HSCs are derived only from the Runx1+Gata1- population. A subset of this population moves from the extra- to intraembryonic region during E7.5-E8.0, where it contributes to the hemogenic endothelium of the dorsal aorta (DA). Migration occurs before the heartbeat is initiated, and it is independent of circulation. This suggests a developmental trajectory from Runx1+ cells in the E7.5 extraembryonic region to definitive HSCs via the hemogenic endothelium.

AB - A large gap exists in our understanding of the course of differentiation from mesoderm to definitive hematopoietic stem cells (HSCs). Previously, we reported that Runx1+ cells in embryonic day 7.5 (E7.5) embryos contribute to the hemogenic endothelium in the E10.5 aorta-gonad-mesonephros (AGM) region and HSCs in the adult bone marrow. Here, we show that two Runx1+ populations subdivided by Gata1 expression exist in E7.5 embryos. The hemogenic endothelium and the HSCs are derived only from the Runx1+Gata1- population. A subset of this population moves from the extra- to intraembryonic region during E7.5-E8.0, where it contributes to the hemogenic endothelium of the dorsal aorta (DA). Migration occurs before the heartbeat is initiated, and it is independent of circulation. This suggests a developmental trajectory from Runx1+ cells in the E7.5 extraembryonic region to definitive HSCs via the hemogenic endothelium.

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Circulation-independent differentiation pathway from extraembryonic mesoderm toward hematopoietic stem cells via hemogenic angioblasts

Abstract

ASJC Scopus subject areas

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