Cloning and characterization of 5′-upstream region of human phospholipase C-β2 gene

Eun Sook Yun, Seung Jae Lee, Myung Jong Kim, Sung Ho Ryu, Pann Ghill Suh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

5′-upstream region of the phospholipase C-β2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5′-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/-13) were identified in the upstream region of PLC-β2 gene. We suggest that the transcription of PLC-β2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalExperimental and Molecular Medicine
Volume33
Issue number2
DOIs
StatePublished - Jun 30 2001

Keywords

  • Cloning
  • Phospholipase C-β2
  • Promoter
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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