Abstract
5′-upstream region of the phospholipase C-β2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5′-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/-13) were identified in the upstream region of PLC-β2 gene. We suggest that the transcription of PLC-β2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.
Original language | English (US) |
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Pages (from-to) | 76-82 |
Number of pages | 7 |
Journal | Experimental and Molecular Medicine |
Volume | 33 |
Issue number | 2 |
DOIs | |
State | Published - Jun 30 2001 |
Keywords
- Cloning
- Phospholipase C-β2
- Promoter
- Transcription
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Clinical Biochemistry